Noninvasive method for monitoring Pneumocystis carinii pneumonia.

Noninvasive method for monitoring Pneumocystis carinii pneumonia.
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DOI:
10.3201/eid0912.030270
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发表时间:
2003-12
影响因子:
11.8
通讯作者:
Cushion MT
Cushion MT
中科院分区:
医学2区
文献类型:
--
作者:
Linke MJ;Rebholz S;Collins M;Tanaka R;Cushion MT

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卡氏肺孢子虫肺炎的进展进行了时间监测和定量的实时聚合酶链反应卡氏肺孢子虫特异性DNA的口腔拭子和肺匀浆从感染的大鼠。DNA水平与大鼠肺中卡氏肺孢子虫的数量相关,如显微镜方法所计数的那样。这份报告是第一个非侵入性的,死前的方法,可用于监测感染的主机随着时间的推移。
The progression of Pneumocystis carinii pneumonia was temporally monitored and quantified by real-time polymerase chain reaction of P. carinii–specific DNA in oral swabs and lung homogenates from infected rats. DNA levels correlated with the number of P. carinii organisms in the rats’ lungs, as enumerated by microscopic methods. This report is the first of a noninvasive, antemortem method that can be used to monitor infection in a host over time.
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发表时间: 1997-11-08
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