Endothelial-Stromal Communication in Murine and Human Corneas.
Endothelial-Stromal Communication in Murine and Human Corneas.
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DOI：
10.1002/ar.24393
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发表时间：
2020-06
期刊：
影响因子：
--
通讯作者：
Espana EM
中科院分区：
文献类型：
--
作者：
Jeang L;Cha BJ;Birk DE;Espana EM
这项研究的目的是识别和描述角膜内皮细胞与其下的后基质的相互作用。在发育3天(P3)、成熟期和出生后30天(P30)的C57BL/6小鼠以及成人角膜中定义了角膜内皮-基质相互作用。从出生后30天(P30)起和出生后30天，用免疫荧光显微镜观察平装和横截面。用鬼臼毒素定位F-肌动蛋白以评估穿过Descemet膜(DM)的细胞突起。通过分析选择性光漂白后钙黄绿素AM染料转移的荧光恢复模式，评价细胞间的动态通讯。内皮-基质相互作用，在发育过程中沿后弹力膜(DM)可见(P3)，仅见于成熟小鼠角膜的外围(P30)。在成熟的人类角膜中，细胞通过DM在周边角膜中延伸。在成熟小鼠和人类中央角膜中，光漂白后的荧光恢复模式表明内皮细胞和内皮细胞之间存在交流。相反，在人的角膜中，观察到两种不同的模式，与内皮-内皮以及基质-内皮的沟通一致。在出生后早期，内皮-基质相互作用存在于整个角膜中。内皮-间质的相互作用局限于成人的角膜周边。内皮-后基质直接接触的存在与角膜内皮细胞通过糖尿病从基质中分离的观点相矛盾，并暗示内皮-基质的沟通可以影响角膜的结构和功能。
The purpose of this study is to identify and characterize interactions of corneal endothelial cells with the underlying posterior stroma. Corneal endothelial - stromal interactions were defined in developing postnatal day 3 (P3) and maturing and postnatal day 30 (P30) C57BL/6 mice as well as in adult human corneas. Flat mounts and cross sections were studied using immunofluorescence microscopy from and postnatal day 30 (P30). F-actin was localized using phalloidin to evaluate cell processes traversing Descemet’s membrane (DM). Dynamic cell - cell communication was evaluated by analyzing the pattern of fluorescence recovery using calcein AM dye transfer after selective photo-bleaching. Endothelial - stromal interactions, seen along Descemet’s membrane (DM) during development (P3), were only found in the periphery of the mature murine cornea (P30). In mature human corneas, cellular extensions through the DM were noted in the peripheral cornea. The pattern of fluorescence recovery after photo-bleaching in both mature mice and human central corneas demonstrated endothelial - endothelial cell communication. In contrast, in the human cornea 2 distinct patterns were observed consistent with endothelial - endothelial as well as stromal - endothelial communication. Endothelial - stromal interactions are present in the entire cornea during early postnatal life. Endothelial-stromal interactions localize to the periphery of the cornea in the adult. The existence of direct endothelial – posterior stromal contact contradicts the conception that corneal endothelial cells are isolated from the stroma by the DM and implies that endothelial - stromal comunication can influence corneal structure and function.
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DOI：
10.1016/0005-2736(74)90085-6
发表时间：
1974-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
PETERS, R;PETERS, J;BAHR, W
通讯作者：
BAHR, W
DOI：
10.1007/b102214
发表时间：
2005-01-01
期刊：
MICROSCOPY TECHNIQUES
影响因子：
--
作者：
Houtsmuller, AB
通讯作者：
Houtsmuller, AB
影响因子：
4.8
作者：
Kawakita, T;Espana, EM;Tseng, SCG
通讯作者：
Tseng, SCG
影响因子：
56.9
作者：
WADE, MH;TROSKO, JE;SCHINDLER, M
通讯作者：
SCHINDLER, M
影响因子：
2.8
作者：
BELL, RS;BOURRET, LA;MANKIN, HJ
通讯作者：
MANKIN, HJ