Structure of the human oxytocinase/insulin-regulated aminopeptidase gene and localization to chromosome 5q21.
Structure of the human oxytocinase/insulin-regulated aminopeptidase gene and localization to chromosome 5q21.
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人催产素酶/胰岛素调节氨肽酶基因的结构和定位于染色体 5q21。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
T. Kristensen
中科院分区：
文献类型：
--
作者：
T. E. Rasmussen;Susana Pedraza;René Hardré;P. G. Laustsen;Ana G. Carríon;T. Kristensen
人催产素/胰岛素调节氨基肽酶(OTase/IRAP)是一种1024个氨基酸的II型完整膜蛋白，主要在脂肪、肌肉和胎盘组织中表达。它被认为主要参与控制分娩的开始，但最近发现大鼠OTase/IRAP也参与了葡萄糖转运体4囊泡在脂肪细胞中的运输。为了更好地理解OTase/IRAP基因调控，我们确定了人类基因的组织。据此，分离并鉴定了3个重叠的基因组克隆。人类OTase/IRAP基因(OTase)全长约75kb，包含18个外显子和17个内含子。与锌离子结合、底物结合和催化有关的谷氨酸氨基肽酶基序：Gaman-(31个氨基酸)-Helah-(18个氨基酸)-E由外显子6和7编码。在翻译起始密码子的上游分别延伸了514和551bp的引物，确定了OTase的一个主要转录起始点和一个次要转录起始点。氯霉素乙酰转移酶报告基因分析显示，CpG启动子/增强子区位于621位核苷酸和主要转录起始点之间。人OTASE通过与特征体细胞杂交种的基因组DNA杂交而被分配到5号染色体上。最后，通过原位杂交将OTase基因和人氨基肽酶A基因分别定位于5q21和4q25。
The human oxytocinase/insulin-regulated aminopeptidase (OTase/IRAP) is a 1024 amino acid type II integral membrane protein that is expressed mainly in fat, muscle and placenta tissues. It has been thought to be involved mainly in the control of onset of labour but recently rat OTase/IRAP was shown to participate in the regulation of glucose transporter isoform 4 vesicle trafficking in adipocytes as well. To approach an understanding of OTase/IRAP gene regulation the organization of the human gene was determined. Accordingly, three overlapping genomic clones were isolated and characterized. The human OTase/IRAP gene (OTASE) was found to span approximately 75 kb containing 18 exons and 17 introns. The gluzincin aminopeptidase motif: GAMEN-(31 amino acids)-HELAH-(18 amino acids)-E associated with Zn2+-binding, substrate binding and catalysis is encoded by exons 6 and 7. A major and a minor transcriptional initiation site in OTASE were identified by primer extension 514 bp and 551 bp, respectively, upstream of the translation start codon. Chloroamphenicol acetyltransferase-reporter assays revealed a functional CpG-rich promoter/enhancer region located between nucleotide -621 and the major transcriptional initiation site. Human OTASE was assigned to chromosome 5 by hybridization to genomic DNA from characterized somatic cell hybrids. Finally, the OTASE and the human aminopeptidase A gene were subchromosomally localized to 5q21 and 4q25, respectively, by in situ hybridization.
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影响因子：
3.1
作者：
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DOI：
--
发表时间：
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期刊：
The Journal of biological chemistry
影响因子：
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作者：
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DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
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作者：
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通讯作者：
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影响因子：
2.9
作者：
Holland,DR;Barclay,PL;Danilewicz,JC;Matthews,BW;James,K
通讯作者：
James,K