Silencing stem cell factor attenuates stemness and inhibits migration of cancer stem cells derived from Lewis lung carcinoma cells
Silencing stem cell factor attenuates stemness and inhibits migration of cancer stem cells derived from Lewis lung carcinoma cells
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沉默干细胞因子可减弱Lewis肺癌细胞来源的癌症干细胞的干性并抑制其迁移
DOI：
10.1007/s13277-015-4577-6
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Luo Feng
中科院分区：
文献类型：
--
作者：
Wang Li;Wang JianTao;Li Zhixi;Liu YanYang;Jiang Ming;Li Yan;Cao Dan;Zhao Maoyuan;Wang Feng;Luo Feng
Stem cell factor (SCF) plays an important role in tumor growth and metastasis. However, the function of SCF in regulating stemness and migration of cancer stem cells (CSCs) remains largely undefined. Here, we report that non-adhesive culture system can enrich and expand CSCs derived from Lewis lung carcinoma (LLC) cells and that the expression level of SCF in CSCs was higher than those in LLC cells. Silencing SCF via short hairpin (sh) RNA lentivirus transduction attenuated sphere formation and inhibited expressions of stemness genes, ALDH1, Sox2, and Oct4 of CSCs in vitro and in vivo. Moreover, SCF-silenced CSCs inhibited the migration and epithelial-mesenchymal transition, with decreased expression of N-cadherin, Vimentin, and increased expression of E-cadherin in vitro and in vivo. Finally, SCF-short hairpin RNA (shRNA) lentivirus transduction suppressed tumorigenicity of CSCs. Taken together, our findings unraveled an important role of SCF in CSCs derived from LLC cells. SCF might serve as a novel target for lung cancer therapy.
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影响因子：
3.7
作者：
Chen SF;Chang YC;Nieh S;Liu CL;Yang CY;Lin YS
通讯作者：
Lin YS
DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
影响因子：
3.4
作者：
Wang L;Guo H;Lin C;Yang L;Wang X
通讯作者：
Wang X
DOI：
--
发表时间：
2010-11
期刊：
The National medical journal of India
影响因子：
--
作者：
Anthony J. Williams;R. Datar;R. Cote
通讯作者：
Anthony J. Williams;R. Datar;R. Cote
来自原发性人类肿瘤的卵巢癌起始细胞的鉴定和表征
影响因子：
11.2
作者：
Zhang, Shu;Balch, Curt;Nephew, Kenneth P.
通讯作者：
Nephew, Kenneth P.