Identification of an intracellular M17 family leucine aminopeptidase that is required for virulence in Staphylococcus aureus.
Identification of an intracellular M17 family leucine aminopeptidase that is required for virulence in Staphylococcus aureus.
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DOI：
10.1016/j.micinf.2012.04.013
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发表时间：
2012-09
影响因子：
5.8
通讯作者：
Shaw LN
中科院分区：
文献类型：
--
作者：
Carroll RK;Robison TM;Rivera FE;Davenport JE;Jonsson IM;Florczyk D;Tarkowski A;Potempa J;Koziel J;Shaw LN
金黄色葡萄球菌是一种高毒力的细菌病原体，能够引起人体多种疾病。由于医院和社区中不断出现高致病性甲氧西林耐药菌株（MRSA），它已成为一个重大的公共卫生问题。金黄色葡萄球菌的毒力由一系列分泌的和细胞壁相关的毒力因子介导，包括毒素、溶血素和蛋白酶。在这项工作中，我们鉴定了一种亮氨酸氨基肽酶（LAP，pepZ），它强烈影响金黄色葡萄球菌的致病能力。 Newman 或 USA300 中 pepZ 基因的破坏导致局部和全身感染模型的毒力急剧减弱。 LAP 是人类巨噬细胞内生存所必需的，基因表达分析表明 pepZ 表达在细胞内环境中最高。我们检查了 LAP 的细胞位置并证明它位于细菌细胞质中。这些结果首次鉴定出影响革兰氏阳性细菌疾病病因的细胞内亮氨酸氨肽酶。
Staphylococcus aureus is a highly virulent bacterial pathogen capable of causing a variety of ailments throughout the human body. It is a major public health concern due to the continued emergence of highly pathogenic methicillin resistant strains (MRSA) both within hospitals and in the community. Virulence in S. aureus is mediated by an array of secreted and cell wall associated virulence factors, including toxins, hemolysins and proteases. In this work we identify a leucine aminopeptidase (LAP, pepZ) that strongly impacts the pathogenic abilities of S. aureus. Disruption of the pepZ gene in either Newman or USA300 resulted in a dramatic attenuation of virulence in both localized and systemic models of infection. LAP is required for survival inside human macrophages and gene expression analysis shows that pepZ expression is highest in the intracellular environment. We examine the cellular location of LAP and demonstrate that it is localized to the bacterial cytosol. These results identify for the first time an intracellular leucine aminopeptidase that influences disease causation in a Gram-positive bacterium.
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DOI：
10.4049/jimmunol.0803663
发表时间：
2009-11-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hearn A;York IA;Rock KL
通讯作者：
Rock KL
影响因子：
11.4
作者：
Alen, C;Sherratt, DJ;Colloms, SD
通讯作者：
Colloms, SD
DOI：
10.1073/pnas.0911813107
发表时间：
2010-02-09
影响因子：
11.1
作者：
McGowan, Sheena;Oellig, Christine A.;Whisstock, James C.
通讯作者：
Whisstock, James C.
影响因子：
2.8
作者：
Bhosle, Manoj;Kadthur, Jayachandra C.;Nandi, Dipankar
通讯作者：
Nandi, Dipankar
影响因子：
5.5
作者：
Maggioli, Gabriela;Acosta, Daniel;Carmona, Carlos
通讯作者：
Carmona, Carlos