Tethering of cellulose synthase to microtubules dampens mechano-induced cytoskeletal organization in Arabidopsis pavement cells.
Tethering of cellulose synthase to microtubules dampens mechano-induced cytoskeletal organization in Arabidopsis pavement cells.
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DOI：
10.1038/s41477-022-01218-7
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发表时间：
2022-09
期刊：
影响因子：
18
通讯作者：
Sampathkumar, Arun
中科院分区：
文献类型：
--
作者：
Schneider, Rene;Ehrhardt, David W.;Meyerowitz, Elliot M.;Sampathkumar, Arun
机械力控制植物和动物的发育，作为模式形成的线索和形态发生的驱动力。在哺乳动物细胞中，位于细胞膜和细胞外基质界面的分子组装体在感知和传递外部机械信号以触发生理反应方面起着重要作用。类似的过程也发生在植物中，但对分子机制及其遗传基础的了解甚少。在这里，我们表明，在质膜的纤维素合成酶复合物（CSC）的数量和运动方向不同，在拟南芥子叶表皮发育的初始阶段，密切反映微管组织。解偶联微管和CSC导致增强的微管共对齐所造成的机械刺激驱动的细胞形状或组织规模的物理扰动。此外，微机械扰动导致CSC从质膜的耗尽，这表明纤维素合酶从质膜的去除和微管对机械刺激的反应之间可能存在联系。两者合计，我们的研究结果表明，纤维素合酶与皮层微管的相互作用形成了一个物理连续的细胞壁，质膜和细胞骨架之间的调节机械响应的细胞骨架。纤维素合成酶的行为及其与微管的相互作用受到机械应力的影响。纤维素合成酶与微管的相互作用阻碍了微管对机械应力的响应。
Mechanical forces control development in plants and animals, acting as cues in pattern formation and as the driving force of morphogenesis. In mammalian cells, molecular assemblies residing at the interface of the cell membrane and the extracellular matrix play an important role in perceiving and transmitting external mechanical signals to trigger physiological responses. Similar processes occur in plants, but there is little understanding of the molecular mechanisms and their genetic basis. Here, we show that the number and movement directions of cellulose synthase complexes (CSCs) at the plasma membrane vary during initial stages of development in the cotyledon epidermis of Arabidopsis, closely mirroring the microtubule organization. Uncoupling microtubules and CSCs resulted in enhanced microtubule co-alignment as caused by mechanical stimuli driven either by cell shape or by tissue-scale physical perturbations. Furthermore, micromechanical perturbation resulted in depletion of CSCs from the plasma membrane, suggesting a possible link between cellulose synthase removal from the plasma membrane and microtubule response to mechanical stimuli. Taken together, our results suggest that the interaction of cellulose synthase with cortical microtubules forms a physical continuum between the cell wall, plasma membrane and the cytoskeleton that modulates the mechano-response of the cytoskeleton. The behaviour of cellulose synthase and its interaction with microtubules are affected by mechanical stress. The interaction of cellulose synthase with microtubules impedes the response of microtubules to mechanical stress.
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影响因子：
11.6
作者：
Burk, DH;Liu, B;Ye, ZH
通讯作者：
Ye, ZH
DOI：
10.1073/pnas.0706569104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Desprez, Thierry;Juraniec, Michal;Vernhettes, Samantha
通讯作者：
Vernhettes, Samantha
DOI：
10.1073/pnas.97.7.3718
发表时间：
2000-03-28
影响因子：
11.1
作者：
Cutler, SR;Ehrhardt, DW;Somerville, CR
通讯作者：
Somerville, CR
影响因子：
20.5
作者：
Bringmann, Martin;Landrein, Benoit;Schudoma, Christian;Hamant, Olivier;Hauser, Marie-Theres;Persson, Staffan
通讯作者：
Persson, Staffan
影响因子：
56.9
作者：
Hamant, Olivier;Heisler, Marcus G.;Traas, Jan
通讯作者：
Traas, Jan