Effects of intermittent pressure imitating rolling manipulation on calcium ion homeostasis in human skeletal muscle cells.
Effects of intermittent pressure imitating rolling manipulation on calcium ion homeostasis in human skeletal muscle cells.
复制标题
间歇压力仿滚动操作对人体骨骼肌细胞钙离子稳态的影响
DOI：
10.1186/s12906-016-1314-7
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发表时间：
2016-08-26
影响因子：
--
通讯作者：
Mason DC
中科院分区：
文献类型：
--
作者：
Zhang H;Liu H;Lin Q;Zhang G;Mason DC
背景细胞内钙离子稳态失衡是骨骼肌损伤的重要病理生理因素之一。这种失衡会导致骨骼肌中钙离子相关生物标志物的代谢发生显著变化，如超氧化物歧化酶(SOD)、丙二醛(MDA)和肌酸激酶(CK)。这些生物标志物的测量可以用来评估人类骨骼肌细胞(HSKMC)损伤的程度。翻转手法是中医最常用的肌筋膜释放手法。这项技术如何改善肌肉损伤的机制尚不清楚。方法以正常HSKMCs为对照组，将损伤HSKMCs分为5组：对照组(CIG)、滚压组(RMG)、滚压+维拉帕米组(RMVG)、静压组(SPG)和静压+维拉帕米组(SPVG)。RMG和RMVG细胞以1.0赫兹的频率周期性地暴露于9.5~12.5N/cm2的IPIRM中10min。SPG和SPVG在12.5N/cm2的持续压力下加载10min。在RMVG组和SPVG组的培养液中加入钙拮抗剂维拉帕米以阻断钙离子内流。结果与正常细胞相比，损伤细胞的SOD活性显著降低，而丙二醛含量和CK活性显著升高。间歇滚动推拿压力(RMG)处理损伤细胞后，细胞内超氧化物歧化酶(SOD)活性显著升高，丙二醛(MDA)含量和CK活性显著降低。在RMVG的培养液中加入钙拮抗剂维拉帕米可抑制上述作用。而无论是否存在维拉帕米，SPG和SPVG静压均不影响损伤肌细胞中的SOD活性、MDA含量和CK活性。结论间歇滚动压力配合手法可通过钙依赖途径减轻HSKMCs的损伤。静压没有导致相同的结果。
BackgroundHomeostasis imbalance of intracellular Ca2+is one of the key pathophysiological factors in skeletal muscle injuries. Such imbalance can cause significant change in the metabolism of Ca2+-related biomarkers in skeletal muscle, such as superoxide dismutase (SOD), malondialdehyde (MDA) and creatine kinase (CK). Measurements of these biomarkers can be used to evaluate the degree of damage to human skeletal muscle cells (HSKMCs) injury. Rolling manipulation is the most popular myofascial release technique in Traditional Chinese Medicine. The mechanism of how this technique works in ameliorating muscle injury is unknown. This study aimed to investigate the possible Ca2+mediated effects of intermittent pressure imitating rolling manipulation (IPIRM) of Traditional Chinese Medicine in the injured HSKMCs.MethodsThe normal HSKMCs was used as control normal group (CNG), while the injured HSKMCs were further divided into five different groups: control injured group (CIG), Rolling manipulation group (RMG), Rolling manipulation-Verapamil group (RMVG), static pressure group (SPG) and static pressure-Verapamil group (SPVG). RMG and RMVG cells were cyclically exposed to 9.5-12.5 N/cm2of IPIRM at a frequency of 1.0 Hz for 10 min. SPG and SPVG were loaded to a continuous pressure of 12.5 N/cm2for 10 min. Verapamil, a calcium antagonist, was added into the culture mediums of both RMVG and SPVG groups to block the influx of calcium ion.ResultCompared with the CNG (normal cells), SOD activity was remarkably decreased while both MDA content and CK activity were significantly increased in the CIG (injured cells). When the injured cells were treated with the intermittent rolling manipulation pressure (RMG), the SOD activity was significantly increased and MDA content and CK activity were remarkably decreased. These effects were suppressed by adding the calcium antagonist Verapamil into the culture medium in RMVG. On the other hand, exposure to static pressure in SPG and SPVG affected neither the SOD activity nor the MDA content and CK activity in the injured muscle cells regardless of the presence of verapamil or not in the culture medium.ConclusionThese data suggest that the intermittent rolling pressure with the manipulation could ameliorate HSKMCs injury through a Ca2+dependent pathway. Static pressure did not lead to the same results.
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