The Role of Dendritic Cell Subsets in Recurrent Spontaneous Abortion and the Regulatory Effect of Baicalin on It.
The Role of Dendritic Cell Subsets in Recurrent Spontaneous Abortion and the Regulatory Effect of Baicalin on It.
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树突状细胞亚群在复发性流产中的作用及黄芩苷的调节作用
DOI：
10.1155/2022/9693064
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发表时间：
2022
影响因子：
4.1
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Lai N;Fu X;Hei G;Song W;Wei R;Zhu X;Guo Q;Zhang Z;Chu C;Xu K;Li X
复发性自然流产（RSA）是妊娠的相关并发症。树突状细胞（DC）的异常活动和分化已被确定参与RSA，但其潜在机制仍不清楚。黄芩苷具有广泛的药理和生物活性。然而，黄芩苷对RSA中DC功能的影响尚未研究。本研究分析了RSA患者外周及母胎界面DC亚群和功能的变化。然后，我们进一步用黄芩苷治疗RSA小鼠，并分析其治疗作用及其机制。结果表明，RSA患者外周血、蜕膜和RSA母胎鼠的DC均被极化为常规DC，其比例与小鼠胚胎吸收率呈正相关。此外，RSA患者和小鼠的DC显示HLA-DR/MHC-II、CD 80和CD 86的表达增加，但CD 274和33 D1的表达降低。黄芩苷可通过抑制STAT 5-ID 2通路，逆转传统DCs向浆细胞样DCs转化，促进功能分子表达，从而减轻RSA小鼠胚胎吸收。我们的研究进一步证明DC在RSA的发病机制中发挥重要作用，黄芩苷可能用于治疗RSA。
Recurrent spontaneous abortion (RSA) is a relevant complication of pregnancy. Aberrant dendritic cell (DC) activities and differentiation have been identified to be involved in RSA, but the underlying mechanisms remain unclear. Baicalin from Radix Scutellariae possesses a wide range of pharmacological and biological activities. However, the effect of baicalin on DC function in RSA has not been investigated. Here, we analyzed the changes of peripheral and maternal-fetal interface DC subsets and function in patients and mice with RSA, respectively. Then, we further treated RSA mice with baicalin and analyzed the therapeutic effect and underlying mechanism. We found that DCs from the peripheral blood and decidua of RSA patients and the maternal-fetal of RSA mice were all polarized to conventional DCs, whose proportion was positively correlated with the mice embryo absorption rate. Moreover, DCs from RSA patients and mice showed increased expression of HLA-DR/MHC-II, CD80, and CD86 but decreased expression of CD274 and 33D1. Importantly, baicalin could alleviate embryo resorption of RSA mice by reversing conventional DCs to plasmacytoid DCs and functional molecule expression via inhibiting the STAT5-ID2 pathway. Our research further proved that DCs play an important role in the pathogenesis of RSA and baicalin might be used for treating RSA.
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影响因子：
3
作者：
Chernykh ER;Leplina OY;Tikhonova MA;Seledtsova NV;Tyrinova TV;Khonina NA;Ostanin AA;Pasman NM
通讯作者：
Pasman NM
影响因子：
3.7
作者：
Kwiatek M;Gęca T;Krzyżanowski A;Malec A;Kwaśniewska A
通讯作者：
Kwaśniewska A
影响因子：
20.3
作者：
Li, Haiyan S.;Yang, Cliff Y.;Watowich, Stephanie S.
通讯作者：
Watowich, Stephanie S.
盐酸水苏碱诱导的 Th1/Th2/Th17/Treg 范式可减少 RU486 诱导流产小鼠的子宫出血
影响因子：
5.4
作者：
Li, Xia;Wang, Bin;Yao, Chengfang
通讯作者：
Yao, Chengfang
影响因子：
20.3
作者：
Redecha, Patricia;van Rooijen, Nico;Girardi, Guillermina
通讯作者：
Girardi, Guillermina