Urea Transporter B and MicroRNA-200c Differ in Kidney Outer Versus Inner Medulla Following Dehydration.
Urea Transporter B and MicroRNA-200c Differ in Kidney Outer Versus Inner Medulla Following Dehydration.
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DOI：
10.1016/j.amjms.2016.06.003
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发表时间：
2016-09
影响因子：
3.1
通讯作者：
Sands, Jeff M.
中科院分区：
文献类型：
--
作者：
Wang, Juan;Wang, Xiaonan H.;Wang, Haidong;Chen, Ling;Klein, Janet D.;Sands, Jeff M.
尿素转运蛋白（UTs）在尿液浓缩和尿素再循环中很重要，UT-B与两者都有关系。在肾脏中，UT-B最初定位于外髓降直血管，最近在内髓降直血管中检测到。内源性microRNAs（miRs）与基因的3 'UTR结合，通常抑制基因的翻译，在基因调控中起着关键作用。小鼠脱水24小时，然后处死。通过PCR和qPCR分析内髓和外髓的microRNA表达，并通过蛋白质印迹分析蛋白质丰度。对小鼠内髓的microRNA测序分析显示，与对照组相比，脱水小鼠的microRNA-200 c增加了40%。对miR-200 c靶点的计算机分析显示，microRNA-200 c可以直接靶向UT-B基因。PCR证实miR-200 c在脱水小鼠的内髓质中上调，而Western blot显示UT-B蛋白丰度在肾脏的相同部分中下调。然而，在外部髓质中，miR-200 c在脱水小鼠中减少，UT-B蛋白在脱水小鼠中增加。这是第一个表明UT-B蛋白和miR-200 c可能各自受到肾脏外髓和内髓内脱水的差异调节。miR-200 c的变化方向与内髓质和外髓质中UT-B蛋白丰度之间的负相关性表明，miR-200 c可能与肾髓质这两个部分中UT-B蛋白的变化相关。
Urea transporters (UTs) are important in urine concentration and in urea recycling, and UT-B has been implicated in both. In kidney, UT-B was originally localized to outer medullary descending vasa recta, and more recently detected in inner medullary descending vasa recta. Endogenously produced microRNAs (miRs) bind to the 3’UTR of genes and generally inhibit their translation, thus playing a pivotal role gene regulation. Mice were dehydrated for 24h then sacrificed. Inner and outer medullas were analyzed by PCR and qPCR for microRNA expression and western blotting for protein abundance. MicroRNA seq analysis of mouse inner medullas showed a 40% increase in microRNA-200c in dehydrated mice compared with controls. An in silico analysis of the targets for miR-200c revealed that microRNA-200c could directly target the gene for UT-B. PCR confirmed that miR-200c is up-regulated in the inner medullas of dehydrated mice while western blot showed that UT-B protein abundance was down-regulated in the same portion of the kidney. However in the outer medulla, miR-200c was reduced and UT-B protein was increased in dehydrated mice. This is the first indication that UT-B protein and miR-200c may each be differentially regulated by dehydration within the kidney outer and inner medulla. The inverse correlation between the direction of change in miR-200c and UT-B protein abundance in both the inner and outer medulla suggests that miR-200c may be associated with the change in UT-B protein in these two portions of the kidney medulla.
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影响因子：
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