Ca2+ imaging in perfused adrenal medullae.
Ca2+ imaging in perfused adrenal medullae.
复制标题
肾上腺髓质灌注 Ca2 成像。
DOI：
10.7888/juoeh.34.163
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
M. Inoue
中科院分区：
文献类型：
--
作者：
A. Warashina;M. Inoue
Ca2+ 成像方法的开发目的是为了探索大范围内肾上腺髓质 (AM) 细胞对突触输入和化学物质的反应的兴奋性变化。通过肾上腺静脉向大鼠和豚鼠的肾上腺髓质逆行加载Ca2+指示剂。对保留在肾上腺中的神经纤维进行电刺激，以诱导 AM 细胞发生突触后反应，并通过将化学物质添加到灌注液中来将化学物质施加到细胞上。通过这种方法，γ-氨基丁酸 (GABA) 被证明可以分别增加豚鼠和大鼠几乎所有 AM 细胞和 40% AM 细胞中的 Ca2+ 信号。
The Ca2+ imaging method was developed to explore changes in excitability in adrenal medullary (AM) cells in a large field in response to synaptic input and chemicals. The adrenal medullae of rats and guinea pigs were retrogradely loaded with Ca2+ indicator through the adrenal vein. Nerve fibers remaining in the adrenal gland were electrically stimulated to induce postsynaptic responses in AM cells, and chemicals were applied to the cells by adding to the perfusate. With this method, gamma-aminobutyric acid (GABA) was shown to increase the Ca2+ signal in almost all and 40% AM cells in guinea pigs and rats, respectively.
关于乙酰胆碱或大鼠肾上腺透壁刺激引起的儿茶酚胺分泌的研究。
DOI：
10.1113/jphysiol.1981.sp013676
发表时间：
1981
期刊：
The Journal of physiology
影响因子：
--
作者：
Wakade,AR
通讯作者：
Wakade,AR