Studies on secretion of catecholamines evoked by acetylcholine or transmural stimulation of the rat adrenal gland.
Studies on secretion of catecholamines evoked by acetylcholine or transmural stimulation of the rat adrenal gland.
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关于乙酰胆碱或大鼠肾上腺透壁刺激引起的儿茶酚胺分泌的研究。
DOI：
10.1113/jphysiol.1981.sp013676
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Wakade,AR
中科院分区：
文献类型：
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作者：
Wakade,AR
1.本文报道了用透壁刺激和乙酰胆碱（ACh）刺激离体灌流大鼠肾上腺，研究肾上腺分泌儿茶酚胺（CA）的方法。2.从4.42 μ M到1.32 mM，CA的分泌几乎与ACh给药呈线性关系。从0.5 - 3 Hz的刺激频率开始，刺激频率增加，刺激频率为10 Hz时，CA的分泌增加;当频率增加到30 Hz时，CA的分泌减少。对透壁刺激的分泌反应最大，持续时间超过1毫秒，60 V。无论刺激持续时间、电压和刺激频率如何，0.31 μ M河豚毒素（TTX）均可阻断透壁刺激诱发的分泌（70 - 95%）。TTX可使ACh诱发的分泌抑制43%。美加明（0.59 mM）治疗后，由任一程序引起的分泌反应被阻断约80%。4.腺苷（0.18 mM），腺苷一磷酸（0.28 mM），或腺苷三磷酸（0.19 mM）降低CA分泌引起的透壁刺激约40%，但对分泌诱导乙酰胆碱没有影响。5.异丙肾上腺素（4.52 μ M）、普萘洛尔（11.58 μ M）、可乐定（13.00 μ M）、酚苄明（3.30 μ M）和4-氨基吡啶（3 mM）均未改变由透壁刺激或ACh诱发的CA分泌。6.用0.25 mM-Ca-Krebs溶液灌注肾上腺完全消除了由透壁刺激引起的CA分泌，但ACh诱导的分泌仍为对照值的30 - 50%。20 mM-Mg阻断了60%的电诱导分泌，但ACh诱发的分泌不受影响。7.用无钙Krebs溶液灌注2小时未完全消除反应。然而，用EGTA（5 mM）处理30分钟完全阻断ACh诱导的分泌。8. La或Mn在阻断CA的经壁诱发分泌方面比ACh诱发分泌更有效。维拉帕米（0.1 mM）对两种方法诱发的分泌均无显着影响。其浓度增加5倍导致约75%的分泌阻断。9. CA分泌的各种离子和试剂的差异效应的基础上，这些化合物的影响神经分泌特性的突触前内脏神经末梢和嗜铬细胞不同的解释。
1. A method of studying the secretion of catecholamines (CA) in the isolated perfused rat adrenal gland by transmural stimulation or by application of acetylcholine (ACh) has been described. 2. Secretion of CA was practically linear in response to ACh administration, starting from 4.42 microM to 1.32 mM. Transmural stimulation enhanced secretion from a stimulation frequency of 0.5‐‐3 Hz; the effect levelled at 10 Hz, and declined as frequency was raised to 30 Hz. The secretory response to transmural stimulation was maximal over 1 msec duration and 60 V. 3. Secretion evoked by transmural stimulation was blocked (70‐95%) by 0.31 microM‐tetrodotoxin (TTX) irrespective of stimulus duration, voltage and frequency of stimulation. Secretion evoked by ACh was depressed 43% by TTX. After mecamylamine (0.59 mM) treatment, secretory response evoked by either procedure was blocked by about 80%. 4. Adenosine (0.18 mM), adenosine monophosphate (0.28 mM), or adenosine triphosphate (0.19 mM) lowered CA secretion evoked by transmural stimulation by about 40%, but had no effect on secretion induced by ACh. 5. Isoprenaline (4.52 microM), propranolol (11.58 microM), clonidine (13.00 microM), phenoxybenzamine (3.30 microM), and 4‐aminopyridine (3 mM) did not modify CA secretion evoked by transmural stimulation or by ACh. 6. Perfusion of the adrenal gland with 0.25 mM‐Ca‐Krebs solution completely abolished CA secretion evoked by transmural stimulation, but ACh‐induced secretion was still 30‐50% of the control value. 20 mM‐Mg blocked electrically induced secretion by 60%, but that evoked by ACh was unaffected. 7. Perfusion with Ca‐free Krebs solution for 2 hr did not completely abolish the response. However, treatment with EGTA (5 mM) for 30 min totally blocked ACh‐induced secretion. 8. La or Mn were more effective in blocking transmurally evoked secretion than ACh‐evoked secretion of CA. Verapamil (0.1 mM) had no significant effect on secretion evoked by either procedure. A 5‐fold increase in its concentration caused about 75% blockade of secretion. 9. Differential effects of various ions and agents on CA secretion are explained on the basis that these compounds affect neurosecretory properties of the presynaptic splanchnic nerve terminals and of chromaffin cells differently.
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乙酰胆碱和儿茶酚胺对豚鼠大肠杆菌平滑肌细胞内钙储存的作用。
DOI：
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发表时间：
1979
期刊：
Journal of Physiology
影响因子：
--
作者：
R. Casteels;L. Raeymaekers
通讯作者：
L. Raeymaekers
有证据表明，分泌性肾上腺嗜铬细胞直接从富含 ATP 的颗粒中释放儿茶酚胺
DOI：
10.1113/jphysiol.1966.sp007863
发表时间：
1966
期刊：
The Journal of Physiology
影响因子：
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作者：
W. Douglas;A. Poisner
通讯作者：
A. Poisner
去甲肾上腺素抑制肾上腺髓质分泌儿茶酚胺。
影响因子：
5
作者：
Y. Gutman;P. Boonyaviroj
通讯作者：
P. Boonyaviroj
镧对肾上腺髓质释放儿茶酚胺的影响
影响因子：
6.1
作者：
J. Borowitz
通讯作者：
J. Borowitz
影响因子：
--
作者：
BOZLER, E
通讯作者：
BOZLER, E