Serologic responses to SARS-CoV-2 infection among hospital staff with mild disease in eastern France.
Serologic responses to SARS-CoV-2 infection among hospital staff with mild disease in eastern France.
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DOI：
10.1016/j.ebiom.2020.102915
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发表时间：
2020-09
期刊：
影响因子：
11.1
通讯作者：
Fontanet A
中科院分区：
文献类型：
--
作者：
Fafi-Kremer S;Bruel T;Madec Y;Grant R;Tondeur L;Grzelak L;Staropoli I;Anna F;Souque P;Fernandes-Pellerin S;Jolly N;Renaudat C;Ungeheuer MN;Schmidt-Mutter C;Collongues N;Bolle A;Velay A;Lefebvre N;Mielcarek M;Meyer N;Rey D;Charneau P;Hoen B;De Seze J;Schwartz O;Fontanet A
轻度SARS-CoV-2感染个体的血清学反应特征不明显。从PCR证实的SARS-CoV-2轻度感染中恢复的医院工作人员使用两种检测方法检测抗SARS-CoV-2抗体：快速免疫诊断检测（99.4%特异性）和S-Flow检测（~99%特异性）。用基于假病毒的测定法测试血清的中和活性。在参与调查的162名医院工作人员中，有160人报告了SARS-CoV-2感染，但不需要住院，并被纳入这些分析。从症状发作到血样采集的中位时间为24天（IQR：21-28，范围13-39）。快速免疫诊断试验在153例（95.6%）样本中检测到抗体，S-Flow试验在159例（99.4%）样本中检测到抗体，在症状发作后18天收集的一份样本中未能检测到抗体（快速试验未在该患者中检测到抗体）。在症状发作后13-20、21-27和28-41天采集的样本中，分别有79%、92%和98%的样本检出中和抗体（NAb）（P = 0.02）。从COVID-19症状出现后13天起，几乎所有医院工作人员的样本中都检测到了SARS-CoV-2抗体。这一发现支持使用血清学检测来诊断从SARS-CoV-2感染中恢复的个体。抗体的中和活性随时间增加。未来的研究将有助于评估恢复患者体液反应的持续性及其相关的中和能力。资助者在研究设计、数据收集、解释或决定提交工作以供出版方面没有任何作用。
The serologic response of individuals with mild forms of SARS-CoV-2 infection is poorly characterized. Hospital staff who had recovered from mild forms of PCR-confirmed SARS-CoV-2 infection were tested for anti-SARS-CoV-2 antibodies using two assays: a rapid immunodiagnostic test (99.4% specificity) and the S-Flow assay (~99% specificity). The neutralizing activity of the sera was tested with a pseudovirus-based assay. Of 162 hospital staff who participated in the investigation, 160 reported SARS-CoV-2 infection that had not required hospital admission and were included in these analyses. The median time from symptom onset to blood sample collection was 24 days (IQR: 21–28, range 13–39). The rapid immunodiagnostic test detected antibodies in 153 (95.6%) of the samples and the S-Flow assay in 159 (99.4%), failing to detect antibodies in one sample collected 18 days after symptom onset (the rapid test did not detect antibodies in that patient). Neutralizing antibodies (NAbs) were detected in 79%, 92% and 98% of samples collected 13–20, 21–27 and 28–41 days after symptom onset, respectively (P = 0.02). Antibodies against SARS-CoV-2 were detected in virtually all hospital staff sampled from 13 days after the onset of COVID-19 symptoms. This finding supports the use of serologic testing for the diagnosis of individuals who have recovered from SARS-CoV-2 infection. The neutralizing activity of the antibodies increased overtime. Future studies will help assess the persistence of the humoral response and its associated neutralization capacity in recovered patients. The funders had no role in study design, data collection, interpretation, or the decision to submit the work for publication.
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影响因子：
2
作者：
Zhao, Jijun;Wang, Qin;Zhang, Jianhua
通讯作者：
Zhang, Jianhua
影响因子：
6.9
作者：
Mo, HY;Zeng, GQ;Zhong, NS
通讯作者：
Zhong, NS
DOI：
10.1016/j.diagmicrobio.2020.115181
发表时间：
2020-12-01
影响因子：
2.9
作者：
Velay, Aurelie;Gallais, Floriane;Fafi-Kremer, Samira
通讯作者：
Fafi-Kremer, Samira
DOI：
10.1016/j.ijid.2014.10.002
发表时间：
2014-12
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
--
作者：
Memish ZA;Assiri AM;Al-Tawfiq JA
通讯作者：
Al-Tawfiq JA
影响因子：
82.9
作者：
Amanat F;Stadlbauer D;Strohmeier S;Nguyen THO;Chromikova V;McMahon M;Jiang K;Arunkumar GA;Jurczyszak D;Polanco J;Bermudez-Gonzalez M;Kleiner G;Aydillo T;Miorin L;Fierer DS;Lugo LA;Kojic EM;Stoever J;Liu STH;Cunningham-Rundles C;Felgner PL;Moran T;García-Sastre A;Caplivski D;Cheng AC;Kedzierska K;Vapalahti O;Hepojoki JM;Simon V;Krammer F
通讯作者：
Krammer F