Middle East respiratory syndrome coronavirus (MERS-CoV) viral shedding in the respiratory tract: an observational analysis with infection control implications.
Middle East respiratory syndrome coronavirus (MERS-CoV) viral shedding in the respiratory tract: an observational analysis with infection control implications.
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DOI：
10.1016/j.ijid.2014.10.002
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Al-Tawfiq JA
中科院分区：
文献类型：
--
作者：
Memish ZA;Assiri AM;Al-Tawfiq JA
自首次描述中东呼吸综合征冠状病毒（MERS-CoV）以来，尚不清楚患者在呼吸道分泌物中传播病毒的时间。因此，我们分析了已确诊MERS CoV感染和无症状阳性接触者中MERS CoV检测阴性时间的可用数据。记录了2012年9月1日至2013年9月31日在沙特阿拉伯王国吉达的沙特阿拉伯病毒学参考实验室收到的呼吸道样本的重复实验室检测数据。使用MERS-CoV的实时RT-PCR测试。根据样本来源、样本类型和MERS-CoV PCR检测结果分析数据。26人（13名患者和13名接触者）进行了重复检测，直到获得阴性检测结果。MERS-CoV病例的大多数样本为气管抽吸物/痰液（p = 0.0006），接触者的大多数样本为鼻和咽拭子（p = 0.0002）。Kaplan-Meier曲线分析显示，接触者清除病毒的时间比患者早得多。在第12天，通过PCR检测，30%的接触者和76%的病例仍然对MERS CoV呈阳性。接触者比患病患者更早清除MERS CoV。这一发现可能与样本类型以及研究的患者类型有关。更多患有显著合并症的患者在显著更长的时间内摆脱病毒。这项研究的结果可能对感染控制指导及其在处理阳性病例的医疗机构中的应用产生重要影响。
Since the first description of Middle East respiratory syndrome coronavirus (MERS-CoV), it has not been known how long patients shed the virus in respiratory secretions. Thus, we analyzed the available data on time to negative MERS-CoV test in patients with confirmed MERS-CoV infection and asymptomatic positive contacts. Data from repeated laboratory testing of respiratory samples received at the Saudi Arabian virology reference laboratory in Jeddah, Kingdom of Saudi Arabia from September 1, 2012 to September 31, 2013 were recorded. A real-time RT-PCR test for MERS-CoV was used. Data were analyzed by origin of sample, sample type, and MERS-CoV PCR test results. Twenty-six individuals (13 patients and 13 contacts) had repeated testing done until a negative test was obtained. Most samples from MERS-CoV cases were tracheal aspirate/sputum (p = 0.0006) and most samples from contacts were nose and throat swabs (p = 0.0002). Kaplan–Meier curve analysis showed that contacts cleared the virus at a much earlier time than patients. On day 12, 30% of contacts and 76% of cases were still positive for MERS-CoV by PCR. Contacts cleared MERS-CoV earlier than ill patients. This finding could be related to the types of sample as well as the types of patient studied. More ill patients with significant comorbidities shed the virus for a significantly longer time. The results of this study could have critical implications for infection control guidance and its application in healthcare facilities handling positive cases.
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影响因子：
15.9
作者：
Al-Tawfiq JA;Memish ZA
通讯作者：
Memish ZA
DOI：
10.1056/nejmoa1306742
发表时间：
2013-08-01
期刊：
The New England journal of medicine
影响因子：
--
作者：
Assiri A;McGeer A;Perl TM;Price CS;Al Rabeeah AA;Cummings DA;Alabdullatif ZN;Assad M;Almulhim A;Makhdoom H;Madani H;Alhakeem R;Al-Tawfiq JA;Cotten M;Watson SJ;Kellam P;Zumla AI;Memish ZA;KSA MERS-CoV Investigation Team
通讯作者：
KSA MERS-CoV Investigation Team
DOI：
10.1016/s1473-3099(13)70154-3
发表时间：
2013-09
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Drosten C;Seilmaier M;Corman VM;Hartmann W;Scheible G;Sack S;Guggemos W;Kallies R;Muth D;Junglen S;Müller MA;Haas W;Guberina H;Röhnisch T;Schmid-Wendtner M;Aldabbagh S;Dittmer U;Gold H;Graf P;Bonin F;Rambaut A;Wendtner CM
通讯作者：
Wendtner CM
影响因子：
11.8
作者：
Al-Tawfiq, Jaffar A.;Hinedi, Kareem;Memish, Ziad A.
通讯作者：
Memish, Ziad A.
影响因子：
12
作者：
Al-Tawfiq, Jaffar A.;Zumla, Alimuddin;Memish, Ziad A.
通讯作者：
Memish, Ziad A.