Development of synaptic connectivity onto interneurons in stratum radiatum in the CA1 region of the rat hippocampus.
Development of synaptic connectivity onto interneurons in stratum radiatum in the CA1 region of the rat hippocampus.
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DOI：
10.1186/1471-2202-13-14
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发表时间：
2012-01-25
期刊：
影响因子：
2.4
通讯作者：
Hanse E
中科院分区：
文献类型：
--
作者：
Riebe I;Hanse E
给定突触前神经元对突触后神经元的放电概率的影响关键取决于连接两个神经元的功能释放位点的数量。确定突触后神经元上的平均功能性突触连接性的一种方式是将动作电位依赖性自发突触电流的幅度与不依赖于动作电位的突触电流的幅度（“minis”）进行比较。用这种方法，它已被发现，平均突触连接之间的突触能CA 3和CA 1锥体细胞增加，从单一的连接在新生大鼠，在年轻的成年大鼠的多个连接。另一方面，γ-氨基丁酸（GABA）能中间神经元在新生大鼠中已经形成到CA 1锥体细胞的多重连接，并且多重GABA能连接的程度被保留到成年。在本研究中，我们研究了海马切片中GABA能CA 1放射层中间神经元的谷氨酸和GABA连接的发展，并将其与CA 1锥体神经元的连接进行了比较。在GABA能中间神经元的CA 1层辐射，无论发育阶段，我们发现，动作电位依赖自发AMPA受体介导的突触电流的平均幅度是相同的大小作为迷你AMPA受体介导的突触电流。这一发现表明，这些GABA能中间神经元，在CA 1锥体神经元相反，在整个发育过程中保持单一的谷氨酸连接。另一方面，对于GABA连接，我们在中间神经元上发现了多个功能性突触连接，就像在锥体细胞上一样。这里提出的结果证实，谷氨酸和GABA突触连接在海马CA 1区的发展非常不同。因此，虽然平均GABA连接在整个发展过程中是多个，谷氨酸连接是单一的早期发展。我们的研究结果进一步表明，谷氨酸突触连接的发展显着不同的锥体细胞和GABA能中间神经元之间的放射层，这样一个给定的突触前谷氨酸能细胞似乎不允许增加其连接到突触后放射层中间神经元，因为它可能会对突触后CA 1锥体细胞。
The impact of a given presynaptic neuron on the firing probability of the postsynaptic neuron critically depends on the number of functional release sites that connect the two neurons. One way of determining the average functional synaptic connectivity onto a postsynaptic neuron is to compare the amplitudes of action potential dependent spontaneous synaptic currents with the amplitude of the synaptic currents that are independent of action potentials ("minis"). With this method it has been found that average synaptic connectivity between glutamatergic CA3 and CA1 pyramidal cells increases from single connections in the neonatal rat, to multiple connections in the young adult rat. On the other hand, γ-aminobutyric acid (GABA)ergic interneurons form multiple connections onto CA1 pyramidal cells already in the neonatal rat, and the degree of multiple GABAergic connectivity is preserved into adulthood. In the present study, we have examined the development of glutamate and GABA connectivity onto GABAergic CA1 stratum radiatum interneurons in the hippocampal slice, and compared this to the connectivity onto CA1 pyramidal neurons. In GABAergic interneurons in the CA1 stratum radiatum, irrespective of developmental stage, we found that the average amplitude of action potential dependent spontaneous AMPA receptor-mediated synaptic currents were of the same magnitude as the mini AMPA receptor mediated synaptic currents. This finding indicates that these GABAergic interneurons, in contrast to the CA1 pyramidal neurons, preserve single glutamate connectivity throughout development. For GABA connectivity, on the other hand, we found multiple functional synaptic connections onto the interneurons, as onto the pyramidal cells. The results presented here confirm that glutamate and GABA synaptic connectivity develop very differently in the hippocampal CA1 region. Thus, whereas average GABA connectivity is multiple throughout the development, glutamate connectivity is unitary early in development. Our results further suggest that the development of glutamate synaptic connectivity differs markedly between pyramidal cells and GABAergic interneurons in stratum radiatum, such that a given presynaptic glutamatergic cell appears not allowed to increase its connectivity onto the postsynaptic stratum radiatum interneuron, as it may do onto the postsynaptic CA1 pyramidal cell.
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DOI：
10.1126/science.1137450
发表时间：
2007-03-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lamsa KP;Heeroma JH;Somogyi P;Rusakov DA;Kullmann DM
通讯作者：
Kullmann DM
单个海马GABA能突触的突触后反应的释放概率依赖性尺度。
DOI：
10.1523/jneurosci.3106-06.2006
发表时间：
2006-11-29
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Biró AA;Holderith NB;Nusser Z
通讯作者：
Nusser Z
影响因子：
3.4
作者：
Gulyás, AI;Tóth, K;Freund, TF
通讯作者：
Freund, TF
影响因子：
64.8
作者：
GULYAS, AI;MILES, R;FREUND, TF
通讯作者：
FREUND, TF
影响因子：
25
作者：
Oertner, TG;Sabatini, BL;Svoboda, K
通讯作者：
Svoboda, K