Physical interaction between Langerhans cells and T-lymphocytes during antigen presentation in vitro.
Physical interaction between Langerhans cells and T-lymphocytes during antigen presentation in vitro.
复制标题
朗格汉斯细胞和 T 淋巴细胞在体外抗原呈递过程中的物理相互作用。
DOI：
10.1111/1523-1747.ep12472117
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Italo Caorsi
中科院分区：
文献类型：
--
作者：
Miguel Concha;Alejandra Vidal;Gladys Garcés;Carlos D. Figueroa;Italo Caorsi
朗格汉斯细胞和T细胞之间的物理相互作用是抗原呈递的基本要求。在这项研究中，我们报告的抗原特异性的物理相互作用，在体外发生的小鼠朗格汉斯细胞和T细胞之间的超微结构特征。用2，4-二硝基苯基-多汁鲎血蓝蛋白脉冲通过淘选方法富集至78%纯度的表皮朗格汉斯细胞，并与用相同抗原在体内引发的同基因T淋巴细胞共孵育。在4 ℃下孵育60分钟后，获得大量由三个或更多个淋巴细胞包围的朗格汉斯细胞构成的缀合物。电子显微镜的共轭物显示，朗格汉斯细胞和T淋巴细胞相互作用的两种类型的接触。I型的特点是糖萼-糖萼相互作用，发生在两个细胞的突起或微绒毛。II型的特点是广泛和紧密的区域之间的密切贴壁朗格汉斯细胞和T淋巴细胞质膜。在这些区域中，存在具有细胞间桥和小的间板连接的区域，高度提示间隙连接。一个电子免疫金程序证明了DNP-LPH抗原的存在下，对I型接触。我们的研究结果表明，I型接触可能代表抗原呈递的位点，而II型接触可能参与抗原呈递过程中保持朗格汉斯细胞和淋巴细胞之间的接触。
Physical interaction between Langerhans cells and T cells is an essential requirement for antigen presentation. In this study we report the ultrastructural characteristics of the antigen-specific physical interactions that occur in vitro between murine Langerhans cells and T cells. Epidermal Langerhans cells enriched to 78% purity by a panning method were pulsed with 2,4-dinitrophenyl-Limulus polyphemus hemocyanin and co-incubated with syngeneic T lymphocytes primed in vivo with the same antigen. A substantial number of conjugates constituted by Langerhans cells surrounded by three or more lymphocytes were obtained after 60 min of incubation at 4 degrees C. Electron microscopy of the conjugates revealed that Langerhans cells and T lymphocytes interacted by two type of contacts. Type I was characterized by glycocalyx-glycocalyx interaction that occurred in relation to protrusions or microvilli of both cells. Type II was characterized by wide and tight areas of close apposition between Langerhans cells and T-lymphocyte plasma membranes. In these areas there were zones with intercellular bridges and small septilaminar junctions highly suggestive of gap junctions. An electron-immunogold procedure demonstrated the presence of DNP-LPH antigen on the type I contact. Our findings suggest that type I contact may represent the locus for antigen presentation whereas the type II contact may be involved in keeping adhesiveness between Langerhans cells and lymphocytes during antigen presentation.
登录
查看更多内容
B 细胞相关的 LFA-1 和 T 细胞相关的 ICAM-1 短暂聚集在相互作用细胞之间的接触区域。
影响因子：
4.3
作者：
Sanders,VM;Vitetta,ES
通讯作者：
Vitetta,ES
影响因子：
--
作者：
E. Vitetta;R. Fernandez-Botran;C. Myers;V. Sanders
通讯作者：
E. Vitetta;R. Fernandez-Botran;C. Myers;V. Sanders
抗原特异性 B 细胞和 T 细胞之间物理相互作用的表征。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Sanders,VM;Snyder,JM;Uhr,JW;Vitetta,ES
通讯作者：
Vitetta,ES
啮齿动物表皮朗格汉斯细胞对 ADP 的组织化学特异性高于对 ATP 和 AMP 的组织化学特异性。
DOI：
10.1111/1523-1747.ep12261031
发表时间：
1984
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Chaker,MB;Tharp,MD;Bergstresser,PR
通讯作者：
Bergstresser,PR
通过免疫化学技术检测表皮朗格汉斯细胞的细胞表面标记。
影响因子：
--
作者：
Bergstresser,PR;Juarez,DV
通讯作者：
Juarez,DV