Human oral cells' response to different endodontic restorative materials: an in vitro study.
Human oral cells' response to different endodontic restorative materials: an in vitro study.
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DOI：
10.1186/s13005-014-0055-4
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发表时间：
2014-12-23
影响因子：
3
通讯作者：
Dammaschke T
中科院分区：
文献类型：
--
作者：
Jung S;Mielert J;Kleinheinz J;Dammaschke T
本研究的目的是比较人成骨细胞和人牙周膜（PDL）细胞与不同牙髓修复材料（如矿物三氧化物聚合物（MTA），Biodentine，银汞合金和复合材料）在20天内的生物学相互作用。根据标准化方案收获、培养人PDL细胞和成骨细胞。按照标准化程序，用相应的表面标志物表征细胞群。在不同的组中，标本的生产特别考虑到恒定的尺寸和体积。Richardson染色后进行形态学观察，并于1d、8d、13 d和20 d后进行细胞计数。所有实验均一式三份进行。使用ANOVA和Tukey检验对结果进行统计学分析（p < 0.05）。形态学分析证明两种骨水泥均具有良好的增殖和细胞附着。一个显著的结果是与MTA接触的PDL细胞的有组织的扩散和平行排列，特别是生物牙本质（细胞在第二细胞层中成熟，与第一细胞层交叉）。从8d开始，Biodentine显示出最高的PDL细胞数量（p < 0.05）。Biodentine和MTA导致成骨细胞和PDL细胞培养物中的细胞密度显著更高。当与对照和骨水泥样品相比时，其他组从第8天起显示出较低的PDL细胞密度，从第13天起显示出较低的成骨细胞密度（p < 0.05）。MTA和Biodentine在与人成骨细胞和牙周膜细胞接触时表现出良好的生物相容性。关于细胞存活和增殖，特别是PDL细胞，Biodentine显示出良好的结果，可以被认为是一种耐受性良好的生物活性牙髓材料。本文的在线版本（doi：10.1186/s13005-014-0055-4）包含补充材料，可供授权用户使用。
The aim of this study was to compare the biological interaction of human osteoblasts and cells of the human periodontal ligament (PDL) with different endodontic restorative material as Mineral Trioxide Aggregate (MTA), Biodentine, amalgam and composite over a time period of 20 days. Human PDL cells and osteoblasts were harvested, cultured and according to standardized protocols. The cell populations were characterized with the corresponding surface markers following standardized procedures. The specimens were produced with special regard to constant dimensions and volume in the different groups. Cell attachment and proliferation were evaluated morphologically after Richardson staining and cell count was performed after 1d, 8d, 13d and 20d. All experiments were performed in triplets. The results were statistically analyzed using the ANOVA- and Tukey-test (p < 0.05). Morphological analysis proved good proliferation and cell attachment in both cements. A remarkable result was the organized spreading and parallel alignment of the PDL cells in contact with MTA and especially Biodentine (cells maturing in a second cell layer crossway to the first one). From 8d onward Biodentine showed the highest quantity of PDL cells (p < 0.05). Biodentine and MTA resulted in a significantly higher cell density in osteoblast and PDL cell culture. The other groups showed a lower PDL cell density from 8d and a lower osteoblast cell density from 13d when compared to control and cement samples (p < 0.05). MTA and Biodentine showed a good biocompatibility in contact with the human osteoblasts and cells of the periodontal ligament. Regarding cell survival and proliferation particularly of PDL cells Biodentine showed good results and can be considered as a well-tolerated bioactive endodontic material. The online version of this article (doi:10.1186/s13005-014-0055-4) contains supplementary material, which is available to authorized users.
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DOI：
10.1590/s1678-77572012000100015
发表时间：
2012-02
期刊：
Journal of applied oral science : revista FOB
影响因子：
--
作者：
Al-Hiyasat AS;Al-Sa'Eed OR;Darmani H
通讯作者：
Darmani H
影响因子：
5
作者：
Grech, L.;Mallia, B.;Camilleri, J.
通讯作者：
Camilleri, J.
影响因子：
5
作者：
Dammaschke, T;Gerth, HUV;Schäfer, E
通讯作者：
Schäfer, E
影响因子：
1.6
作者：
Bodrumlu, Emre
通讯作者：
Bodrumlu, Emre
影响因子：
4.2
作者：
DORN, SO;GARTNER, AH
通讯作者：
GARTNER, AH