Quality of cellular attachment to various root-end filling materials.

Quality of cellular attachment to various root-end filling materials.
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DOI:
10.1590/s1678-77572012000100015
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发表时间:
2012-02
期刊:
Journal of applied oral science : revista FOB
影响因子:
--
通讯作者:
Darmani H
Darmani H
中科院分区:
其他
文献类型:
--
作者:
Al-Hiyasat AS;Al-Sa'Eed OR;Darmani H

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本研究调查了6种根端充填材料的细胞附着情况,作为材料生物相容性的指标。 在根片中制备I类逆行腔,用测试材料填充这些腔,并与Balb/C 3 T3成纤维细胞孵育24 h。根切片与空腔留下空作为对照。然后将根切片进行扫描电子显微镜处理,并通过观察细胞的形状、铺展和膜轮廓来观察以评估细胞附着的质量。 在MTA和Geristore表面观察到最好的细胞附着:细胞表现出特征性的细长成纤维细胞形态,其表面具有板状伪足、丝状伪足、大泡和微绒毛的突起,反映了对材料的良好附着。成纤维细胞在EBA、Super EBA、KetacFil和Retroplast表面附着较差。此外,细胞没有很好地附着在牙齿结构旁边的EBA和Super EBA。 本研究表明,细胞附着不同的根端填充材料的最佳细胞附着MTA和Geristore的表面的变化。EBA和Super EBA、Ketac Fil和Retroplast的附着力较差。
This study investigated cellular attachment to 6 root-end filling materials as a measure of the biocompatibility of the materials. Class I retrograde cavities were prepared in root slices and these cavities were filled with the test materials, and incubated with Balb/C 3T3 fibroblasts for 24 h. Root slices with the cavities left empty served as the controls. The root slices were then processed for scanning electron microscopy, and were viewed to assess the quality of cellular attachment by observing the shape of cells, spread, and membrane outline. The best cellular attachment was observed at MTA and Geristore surfaces: cells exhibited characteristic elongated fibroblastic morphology, with projections of lamellipodia, filopodia, blebs, and microvilli from their surfaces, reflecting good attachment to the material. Fibroblasts attached poorly to the surfaces of IRM, Super EBA, KetacFil and Retroplast. Furthermore, the cells did not attach well to the tooth structure next to IRM and Super EBA. The present study demonstrated a variation in cellular attachment to different root-end filling materials with the best cellular attachment to the surfaces of MTA and Geristore. IRM and Super EBA, Ketac Fil and Retroplast rendered poor attachment.
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