Approaches and Tools to Study the Roles of Juvenile Hormones in Controlling Insect Biology.
Approaches and Tools to Study the Roles of Juvenile Hormones in Controlling Insect Biology.
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DOI：
10.3390/insects11120858
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发表时间：
2020-12-03
期刊：
影响因子：
3
通讯作者：
Nouzova M
中科院分区：
文献类型：
--
作者：
Noriega FG;Nouzova M
保幼激素（JH）在昆虫发育和繁殖过程中发挥着至关重要的作用。 JH 的众多作用引发了多个基本科学问题，以及杀虫内分泌干扰物的开发前景。人们越来越需要识别和量化内源性 JH 的方法。这些亲脂性化合物的低滴度和使用困难常常阻碍 JH 生物学的研究。在本文中，我们回顾了有关从昆虫样本中检测和定量 JH、开发操纵 JH 滴度的方法以及使用下一代工具来调节 JH 稳态的现有信息。保幼激素（JH）是一组由幼体合成的倍半萜类化合物。它们在昆虫发育和繁殖过程中发挥着关键作用。为了研究 JH 控制的过程，研究人员需要识别和量化内源 JH 的方法以及可用于在体外和体内增加或减少 JH 滴度的工具。 JH 的亲脂性加上内源滴度低，使得处理和定量具有挑战性。通过局部应用 JH 类似物（例如烯虫酯）可以轻松提高昆虫的 JH 滴度。另一方面，通过实验降低 JH 滴度则更加困难。基于 RNA 干扰和基于 CRISPR/Cas9 的基因组编辑的进展，已经建立了调节 JH 稳态的新方法。本综述将总结以下方面的当前进展：（1）昆虫样本中 JH 的检测和定量； (2) 操纵 JH 滴度的方法； (3) 调节 JH 稳态的下一代工具。
The juvenile hormones (JHs) play critical roles during insect development and reproduction. The numerous effects of JHs have generated multiple basic scientific questions, as well as prospects for the development of insecticidal endocrine disruptors. There is an increasing need for methods to identify and quantify endogenous JHs. The low titers and difficulties in working with these lipophilic compounds have often hindered the study of JH biology. In this article, we review the existing information on the detection and quantification of JH from insect samples, the development of approaches to manipulate JH titers, and the use of next-generation tools to modulate JH homeostasis. The juvenile hormones (JHs) are a group of sesquiterpenoids synthesized by the corpora allata. They play critical roles during insect development and reproduction. To study processes that are controlled by JH, researchers need methods to identify and quantify endogenous JHs and tools that can be used to increase or decrease JH titers in vitro and in vivo. The lipophilic nature of JHs, coupled with the low endogenous titers, make handling and quantification challenging. JH titers in insects can easily be increased by the topical application of JH analogs, such as methoprene. On the other hand, experimentally reducing JH titers has been more difficult. New approaches to modulate JH homeostasis have been established based on advances in RNA interference and CRISPR/Cas9-based genome editing. This review will summarize current advances in: (1) the detection and quantification of JHs from insect samples; (2) approaches to manipulating JH titers; and (3) next-generation tools to modulate JH homeostasis.
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影响因子：
9.2
作者：
Lee, Sang Soo;Ding, Yike;Adams, Michael E.
通讯作者：
Adams, Michael E.
DOI：
10.1073/pnas.1116123109
发表时间：
2011-12-27
影响因子：
11.1
作者：
Charles, Jean-Philippe;Iwema, Thomas;Jindra, Marek
通讯作者：
Jindra, Marek
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
通过反式编码的小 RNA 和宿主因子 RNase III 进行 CRISPR RNA 成熟。
影响因子：
64.8
作者：
Deltcheva, Elitza;Chylinski, Krzysztof;Sharma, Cynthia M.;Gonzales, Karine;Chao, Yanjie;Pirzada, Zaid A.;Eckert, Maria R.;Vogel, Joerg;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
DOI：
10.1016/s0021-9673(00)81664-7
发表时间：
1981-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
BERGOT, BJ;RATCLIFF, M;SCHOOLEY, DA
通讯作者：
SCHOOLEY, DA