Local accessory gene sharing among Egyptian Campylobacter potentially promotes the spread of antimicrobial resistance.
Local accessory gene sharing among Egyptian Campylobacter potentially promotes the spread of antimicrobial resistance.
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DOI：
10.1099/mgen.0.000834
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发表时间：
2022-06
影响因子：
3.9
通讯作者：
Elhadidy, Mohamed
中科院分区：
文献类型：
--
作者：
Mouftah, Shaimaa F.;Pascoe, Ben;Calland, Jessica K.;Mourkas, Evangelos;Tonkin, Naomi;Lefevre, Charlotte;Deuker, Danielle;Smith, Sunny;Wickenden, Harry;Hitchings, Matthew D.;Sheppard, Samuel K.;Elhadidy, Mohamed
弯曲杆菌 腹泻是全世界细菌性胃肠炎的最常见原因，腹泻病是低收入和中等收入国家儿童发病、生长迟缓和死亡的主要原因。尽管有证据表明疾病流行病学的高发病率和差异，但来自发展中国家研究的基因组数据有限。在这项研究中，我们的目标是量化当地和全球人群的基因共享程度。我们描述了一个收集的遗传多样性和附属基因组的内容， 弯曲杆菌 分离自埃及开罗大都市区。共计112个 弯曲杆菌 从肉鸡尸体（n=31）、牛奶和乳制品（n=24）和患有胃肠炎的患者（n=57）中收集分离物。在最常见的序列类型（ST）中，我们确定了全球传播的宿主多面手ST-21克隆复合体（CC 21）和家禽专家CC 206，CC 464和CC 48。值得注意的是，CC 45和养牛专家CC 42的代表人数不足，CC 61完全缺席。核心和附属基因组共享之间进行了比较，从埃及和英国（牛津大学）可比的收集分离。来自同一国家的分离株之间的谱系特异性附件基因组共享显著较高，特别是CC 21，这表明了更大的地方地理聚集性。与此相反，没有地理聚类的核心或辅助基因组的CC 828，表明一个高度混合的人口。更大比例的 结肠弯曲菌 分离株是多重耐药的， 空肠弯曲杆菌我们的研究结果表明，在埃及菌株之间有更多的辅助基因的水平转移。这对于控制这种重要病原体中抗菌素耐药性的传播具有重要意义。
Campylobacter is the most common cause of bacterial gastroenteritis worldwide, and diarrhoeal disease is a major cause of child morbidity, growth faltering and mortality in low- and middle-income countries. Despite evidence of high incidence and differences in disease epidemiology, there is limited genomic data from studies in developing countries. In this study, we aimed to quantify the extent of gene sharing in local and global populations. We characterized the genetic diversity and accessory-genome content of a collection of Campylobacter isolates from the Cairo metropolitan area, Egypt. In total, 112 Campylobacter isolates were collected from broiler carcasses (n=31), milk and dairy products (n=24), and patients suffering from gastroenteritis (n=57). Among the most common sequence types (STs), we identified the globally disseminated host generalist ST-21 clonal complex (CC21) and the poultry specialists CC206, CC464 and CC48. Notably, CC45 and the cattle-specialist CC42 were under-represented, with a total absence of CC61. Core- and accessory-genome sharing was compared among isolates from Egypt and a comparable collection from the UK (Oxford). Lineage-specific accessory-genome sharing was significantly higher among isolates from the same country, particularly CC21, which demonstrated greater local geographical clustering. In contrast, no geographical clustering was noted in either the core or accessory genome of CC828, suggesting a highly admixed population. A greater proportion of Campylobacter coli isolates were multidrug resistant compared to Campylobacter jejuni . Our results suggest that there is more horizontal transfer of accessory genes between strains in Egypt. This has strong implications for controlling the spread of antimicrobial resistance among this important pathogen.
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影响因子：
11.8
作者：
Coker AO;Isokpehi RD;Thomas BN;Amisu KO;Obi CL
通讯作者：
Obi CL
DOI：
10.1093/cid/ciw542
发表时间：
2016-11-01
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Amour C;Gratz J;Mduma E;Svensen E;Rogawski ET;McGrath M;Seidman JC;McCormick BJ;Shrestha S;Samie A;Mahfuz M;Qureshi S;Hotwani A;Babji S;Trigoso DR;Lima AA;Bodhidatta L;Bessong P;Ahmed T;Shakoor S;Kang G;Kosek M;Guerrant RL;Lang D;Gottlieb M;Houpt ER;Platts-Mills JA;Etiology, Risk Factors, and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development Project (MAL-ED) Network Investigators
通讯作者：
Etiology, Risk Factors, and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development Project (MAL-ED) Network Investigators
通才弯曲杆菌菌株中快速的宿主切换侵蚀了追踪人类感染的信号。
DOI：
10.1038/ismej.2015.149
发表时间：
2016-03
期刊：
The ISME journal
影响因子：
--
作者：
Dearlove BL;Cody AJ;Pascoe B;Méric G;Wilson DJ;Sheppard SK
通讯作者：
Sheppard SK
影响因子：
2.1
作者：
Battle SE;Rello J;Hauser AR
通讯作者：
Hauser AR
影响因子：
3.9
作者：
Argimón S;Abudahab K;Goater RJE;Fedosejev A;Bhai J;Glasner C;Feil EJ;Holden MTG;Yeats CA;Grundmann H;Spratt BG;Aanensen DM
通讯作者：
Aanensen DM