Use of extremely short Förster resonance energy transfer probes in real-time polymerase chain reaction.
Use of extremely short Förster resonance energy transfer probes in real-time polymerase chain reaction.
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DOI：
10.1093/nar/gkt782
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发表时间：
2013-11
影响因子：
14.9
通讯作者：
Kutyavin IV
中科院分区：
文献类型：
--
作者：
Kutyavin IV
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Described in the article is a new approach for the sequence-specific detection of nucleic acids in real-time polymerase chain reaction (PCR) using fluorescently labeled oligonucleotide probes. The method is based on the production of PCR amplicons, which fold into dumbbell-like secondary structures carrying a specially designed ‘probe-luring’ sequence at their 5′ ends. Hybridization of this sequence to a complementary ‘anchoring’ tail introduced at the 3′ end of a fluorescent probe enables the probe to bind to its target during PCR, and the subsequent probe cleavage results in the florescence signal. As it has been shown in the study, this amplicon-endorsed and guided formation of the probe-target duplex allows the use of extremely short oligonucleotide probes, up to tetranucleotides in length. In particular, the short length of the fluorescent probes makes possible the development of a ‘universal’ probe inventory that is relatively small in size but represents all possible sequence variations. The unparalleled cost-effectiveness of the inventory approach is discussed. Despite the short length of the probes, this new method, named Angler real-time PCR, remains highly sequence specific, and the results of the study indicate that it can be effectively used for quantitative PCR and the detection of polymorphic variations.
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DOI：
10.1073/pnas.85.20.7652
发表时间：
1988-10-01
影响因子：
11.1
作者：
GYLLENSTEN, UB;ERLICH, HA
通讯作者：
ERLICH, HA
DOI：
10.1073/pnas.85.24.9436
发表时间：
1988-12-01
影响因子：
11.1
作者：
INNIS, MA;MYAMBO, KB;BROW, MAD
通讯作者：
BROW, MAD
影响因子：
4.7
作者：
LOKHOV, SG;PODYMINOGIN, MA;ZARYTOVA, VP
通讯作者：
ZARYTOVA, VP
影响因子：
14.9
作者：
Kutyavin IV
通讯作者：
Kutyavin IV
影响因子：
56.9
作者：
Belgrader, P;Benett, W;Milanovich, F
通讯作者：
Milanovich, F