Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus.
Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus.
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核多角体病毒感染家蚕差异表达 microRNA 的全基因组分析
DOI：
10.1371/journal.pone.0165865
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Chen T
中科院分区：
文献类型：
--
作者：
Wu P;Jiang X;Guo X;Li L;Chen T
家蚕核型多角体病毒(BmNPV)是威胁蚕业发展和可持续发展的主要病原。由于microRNAs(MiRNAs)已被证明在宿主-病原菌相互作用中发挥重要作用，本研究探讨了BmNPV感染对家蚕microRNAs表达的影响。为了实现这一点，我们构建了两个来自BmNPV感染和未感染幼虫的小RNA文库并进行了深度测序。结果表明，家蚕感染BmNPV后，有38个miRNAs存在差异表达。基于GO分析，发现它们预测的靶基因参与多种功能，如结合、催化、病毒粒子和对刺激的免疫反应，这表明它们在宿主-病毒相互作用中可能发挥作用。利用双荧光素酶报告基因分析，我们证实了BMO-miR-277-5p在家蚕感染BmNPV幼虫中上调的靶向DNA胞嘧啶-5甲基转移酶(DNMT2)基因，该基因可能在家蚕与BmNPV的相互作用中发挥潜在作用。这些结果为探索家蚕与BmNPV的相互作用机制提供了新的思路。
Bombyx mori nucleopolyhedrosis virus (BmNPV) is a major pathogen that threatens the growth and sustainability of the sericulture industry. Since microRNAs (miRNAs) have been shown to play important roles in host-pathogen interactions, in this study we investigated the effects of BmNPV infection on silkworm microRNAs expression profile. To achieve this, we constructed and deep-sequenced two small RNA libraries generated from BmNPV infected and un-infected larvae. The results revealed that 38 silkworm miRNAs were differentially expressed after BmNPV infection. Based on the GO analysis, their predicted target genes were found to be involved in diverse functions such as binding, catalytic, virion and immune response to stimulus suggesting their potential roles in host-virus interactions. Using the dual-luciferase reporter assay, we confirmed that Bmo-miR-277-5p, up-regulated in BmNPV-infected larvae, targeted the B. mori DNA cytosine-5 methyltransferase (Dnmt2) gene which may play potential role in silkworm-BmNPV interaction. These results provide new insights into exploring the interaction mechanism between silkworm and BmNPV.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
2.6
作者：
Campbell CL;Harrison T;Hess AM;Ebel GD
通讯作者：
Ebel GD
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
4.1
作者：
Esslinger SM;Schwalb B;Helfer S;Michalik KM;Witte H;Maier KC;Martin D;Michalke B;Tresch A;Cramer P;Förstemann K
通讯作者：
Förstemann K