Juvenile hormone action through a defined enhancer motif to modulate ecdysteroid-activation of natural core promoters.
Juvenile hormone action through a defined enhancer motif to modulate ecdysteroid-activation of natural core promoters.
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DOI：
10.1016/j.cbpb.2011.11.008
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发表时间：
2012-03
影响因子：
2.2
通讯作者：
Wu, Wen-Hui
中科院分区：
文献类型：
--
作者：
Jones, Grace;Jones, Davy;Fang, Fang;Xu, Yong;New, David;Wu, Wen-Hui
我们已经建立了激素作用的模型系统，在Sf 9细胞转染系统中，使用定义的增强子基序和变态相关基因的天然核心启动子。DR 1增强子是蜕皮激素受体/超气门异源二聚体的既定DNA结合位点，是20-OH蜕皮激素转录激活所必需的。对于这种激活的转录，靠近TATA盒5′的天然序列是必需的。保幼激素III的共处理强烈抑制类固醇激活的转录。然而，在缺乏位于TATA盒5′端的序列的情况下，与单独的20-羟基蜕皮激素相比，保幼激素的共处理反而增加了转录。通过与保幼激素共处理激活的这种敏感性可以转移到相关的，但在其他方面无反应的，六聚体核心启动子简单地通过转移到无反应的启动子的五个碱基的转录起始位点（ACAGT）从响应六聚体基因。这些是第一次报告，JH行动的方向20-OH蜕皮激素激活的转录可以逆转的核心启动子的序列的删除，和保幼激素的调节作用可以转移到一个不同的基因通过转移的转录起始位点基序。
We have established a model system of hormone action, in an Sf9 cell transfection system, using defined enhancer motifs and natural core promoters of metamorphosis-associated genes. The DR1 enhancer, that is an established DNA binding site for the ecdysone receptor/ultraspiracle heterodimer, was necessary for transcriptional activation by 20-OH ecdysone. For this activated transcription, a natural sequence closely 5′ to the TATA box is necessary. Cotreatment with juvenile hormone III strongly suppressed the steroid activation of transcription. However, in the absence of the sequence located closely 5′ to the TATA box, cotreatment with juvenile hormone instead increased transcription over that occurring due to 20-hydroxy-ecdysone alone. This sensitivity to activation by cotreatment with juvenile hormone could be transferred to a related, but otherwise unresponsive, hexamerin core promoter simply by transferring to the unresponsive promoter the five base transcription start site (ACAGT) from the responsive hexamerin gene. These are the first reports that the direction of JH action on 20-OH ecdysone-activated transcription can be reversed by removal of a sequence at the core promoter, and that modulatory action of juvenile hormone can be transferred to a different gene by transferring the transcription start site motif.
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