Effect of prenatal methylmercury exposure on neurobehavioral development in male mice: comparison between methylmercury in fish and methylmercury chloride added to diets
Effect of prenatal methylmercury exposure on neurobehavioral development in male mice: comparison between methylmercury in fish and methylmercury chloride added to diets
复制标题
产前甲基汞暴露对雄性小鼠神经行为发育的影响：鱼中甲基汞与饮食中添加氯化甲基汞的比较
DOI：
10.2131/fts.2.67
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
H. Satoh
中科院分区：
文献类型：
--
作者：
Miyuki Iwai;M. Yamashita;N. Kurokawa;Kunihiko Nakai;Mitsuharu Ishida;A. Naganuma;H. Satoh
虽然人类接触甲基汞的主要来源是食用受甲基汞污染的鱼类，但尚不清楚甲基汞对鱼类的毒性是否与实验性添加到饮食中的氯化甲基汞（MeHgCl）相同。我们研究了发育和行为的影响，甲基汞来自鱼类和MeHgCl添加到各种饮食在产前期间的小鼠GD 0至GD 17。将7 - 9只雌性C57 BL/6 NCr小鼠分配到以下暴露组中的每一个：对照组（CL）、CL+MeHgCl（CL+MeHg，1.6 mgHg/kg）、低MeHg金枪鱼（LT，0.2 mgHg/kg）、LT+MeHgCl（LT+MeHg，1.6 mgHg/kg）和高MeHg金枪鱼（HT，1.6 mgHg/kg）。在幼仔中，与其他三组相比，CL+MeHg和LT中的MeHg暴露分别使体重降低和升高。在神经发育测试中，除CL组外，其他4组翻正反射均较CL组有易化发育。HT的悬崖回避反应比CL+MeHg、LT和LT+MeHg慢。在水迷宫实验中，雄性HT的游泳速度比CL慢，而雌性HT的游泳速度与CL无明显差异。LT+MeHg组的男性从旋转杆上跌落的潜伏期显著短于LT组，而女性则无显著差异。我们的研究结果表明，来自鱼类和试剂甲基汞的甲基汞的毒理学概况有些不同的可能性。我们的研究结果还提供了证据表明，男性比女性更容易受到产前甲基汞暴露。
While the primary source of human MeHg exposure is the consumption of fish contaminated with MeHg, it is unknown whether the toxicity of MeHg in fish is equivalent to that of MeHg chloride (MeHgCl) experimentally added to the diet. We investigated developmental and behavioral effects of MeHg derived from fish and MeHgCl added to various diets during the prenatal period in mice from GD 0 to GD 17. From 7 to 9 female C57BL/6NCr mice were assigned to each of the following exposure groups: Control (CL), CL+MeHgCl (CL+MeHg, 1.6 mgHg/kg), low MeHg tuna (LT, 0.2 mgHg/kg), LT+MeHgCl (LT+MeHg, 1.6 mgHg/kg), and high MeHg tuna (HT, 1.6 mgHg/kg). In pups, body weight was depressed and elevated by MeHg exposure in the CL+MeHg and the LT, respectively, compared with other three groups. In neurodevelopmental test, the righting reflex of 4 groups other than CL showed the facilitated developments compared to the CL. The cliff avoidance of the HT developed slower than in the CL+MeHg, LT and LT+MeHg. In water maze test, the swimming speed of the HT decreased in comparison with the CL in males but not females. The latency until falling from a rotating rod of the LT+MeHg was significantly shorter than that of the LT in males but not females. Our results are suggesting the possibility that the toxicological profiles of MeHg derived from fish and reagent MeHg are somewhat different. Our findings also provide evidence that males are more susceptible than females to prenatal MeHg exposure.
影响因子：
8.3
作者：
Grandjean, P;Weihe, P;Debes, F
通讯作者：
Debes, F