Collagen organization regulates stretch-initiated pain-related neuronal signals in vitro: Implications for structure-function relationships in innervated ligaments.
Collagen organization regulates stretch-initiated pain-related neuronal signals in vitro: Implications for structure-function relationships in innervated ligaments.
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胶原组织在体外调节拉伸引发的疼痛相关神经信号：对受神经支配的韧带结构功能关系的影响。
DOI：
10.1002/jor.23657
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Winkelstein,BethA
中科院分区：
文献类型：
--
作者：
Zhang,Sijia;Singh,Sagar;Winkelstein,BethA
脊柱小关节囊（一种具有异质胶原组织的受神经支配的韧带）的损伤会产生疼痛。虽然机械小关节创伤激活嵌入传入，目前尚不清楚，如果，以及如何，其韧带的细胞外微观结构的变化影响感觉传导的机械输入的疼痛。为了研究宏观变形对具有不同组织的胶原基质中的传入的影响，使用体外神经元-胶原构建体（NCC）模型。具有随机组织或平行排列的胶原纤维的NCC用于模拟小关节囊韧带中的不同微观结构。胚胎大鼠背根神经节（DRG）被包裹在NCC中，轴突生长是均匀的，在所有方向的随机NCC中，但平行对齐的NCC。NCC接受了单轴拉伸（0.25 ± 0.06应变），对应于诱发疼痛的亚失效关节突囊应变。测量了宏观NCC力学，并在第1天测定了磷酸化细胞外信号调节激酶（pERK）和神经递质P物质（SP）的轴突表达，以评估神经元活化和伤害感受。拉伸显著上调pERK在随机和对齐凝胶中的表达（p< 0.001），对齐凝胶中pERK的增加显著高于（p= 0.013）随机NCC。这种增加可能与对齐的NCC中更高的峰值力（p= 0.025）和更强的轴突对齐（p< 0.001）与拉伸方向有关。相比之下，无论胶原组织如何，伸展的NCC中SP表达更高（p< 0.001）。这些发现表明，胶原组织差异调节疼痛相关的神经元信号传导，并支持韧带组织的结构异质性介导感觉功能。© 2017年骨科研究学会。由威利期刊公司出版J Orthop Res 36：770-777，2018。
Injury to the spinal facet capsule, an innervated ligament with heterogeneous collagen organization, produces pain. Although mechanical facet joint trauma activates embedded afferents, it is unclear if, and how, the varied extracellular microstructure of its ligament affects sensory transduction for pain from mechanical inputs. To investigate the effects of macroscopic deformations on afferents in collagen matrices with different organizations, an in vitro neuron‐collagen construct (NCC) model was used. NCCs with either randomly organized or parallel aligned collagen fibers were used to mimic the varied microstructure in the facet capsular ligament. Embryonic rat dorsal root ganglia (DRG) were encapsulated in the NCCs; axonal outgrowth was uniform and in all directions in random NCCs, but parallel in aligned NCCs. NCCs underwent uniaxial stretch (0.25 ± 0.06 strain) corresponding to sub‐failure facet capsule strains that induce pain. Macroscopic NCC mechanics were measured and axonal expression of phosphorylated extracellular signal‐regulated kinase (pERK) and the neurotransmitter substance P (SP) was assayed at 1 day to assess neuronal activation and nociception. Stretch significantly upregulated pERK expression in both random and aligned gels (p< 0.001), with the increase in pERK being significantly higher (p= 0.013) in aligned than in random NCCs. That increase likely relates to the higher peak force (p= 0.025) and stronger axon alignment (p< 0.001) with stretch direction in the aligned NCCs. In contrast, SP expression was greater in stretched NCCs (p< 0.001) regardless of collagen organization. These findings suggest that collagen organization differentially modulates pain‐related neuronal signaling and support structural heterogeneity of ligament tissue as mediating sensory function. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:770–777, 2018.
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颞下颌关节综合症和咬合。
DOI：
10.1016/0022-3913(71)90234-4
发表时间：
1971
期刊：
The Journal of prosthetic dentistry
影响因子：
--
作者：
U. Posselt
通讯作者：
U. Posselt
咬合和下颌功能障碍：对因咀嚼系统功能障碍而转诊的患者的临床研究。
DOI：
--
发表时间：
1985
期刊：
The Journal of prosthetic dentistry (Print)
影响因子：
--
作者：
B. Droukas;C. Lindee;G. E. Carlsson
通讯作者：
G. E. Carlsson
肌面疼痛功能障碍：短脸综合征的一种表现。
DOI：
--
发表时间：
1979
期刊：
The Journal of prosthetic dentistry (Print)
影响因子：
--
作者：
Joseph E. Van Sickels;Joseph E. Van Sickels;David W. Ivey;David W. Ivey
通讯作者：
David W. Ivey
随机人群的下颌功能障碍症状
DOI：
--
发表时间：
1983
期刊：
影响因子：
--
作者：
C. Greene;C. Turner;D. Laskin
通讯作者：
D. Laskin
影响因子：
2.6
作者：
M. Janson;A. Hasund
通讯作者：
A. Hasund