The Clinical and Medicolegal Analysis of Electrical Shocked Rats: Based on the Serological and Histological Methods.
The Clinical and Medicolegal Analysis of Electrical Shocked Rats: Based on the Serological and Histological Methods.
复制标题
电击大鼠的临床和法医学分析：基于血清学和组织学方法
DOI：
10.1155/2016/4896319
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发表时间：
2016
影响因子：
--
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Liu H;Wang Q;Zhao Z;Xie Y;Ding S;Wang Z
本研究旨在探讨电击后心脏和肝脏组织的血清学和组织学变化。血清学方法检测CK-MB、ALT、AMS指标。此外，仔细测量Bcl-2、Bax和Hsp-60的表达水平。采用110 V交流电电击大鼠建立电损伤模型。取大鼠血液进行生化指标分析。采用免疫组化法检测Bcl-2、Bax和Hsp-60的表达，评价心脏和肝脏的病理变化程度。电击伤组CK-MB在电击后0.5h迅速达到峰值。此外，电损伤后心脏和肝脏组织中Bcl-2、Bax和Hsp-60的水平呈规律性变化，与对照组相比有明显差异。电击后CK-MB、ALT、AMS有规律的变化，这些结果为临床和法医学实践提供了有意义的信息。本研究为无明显电烧伤的电损伤的评估提供了依据。此外，Bcl-2/Bax和Hsp-60的研究结果也有助于病理诊断和鉴定生前和死后的电损伤。
This research was aimed at discovering the serological and histological changes in cardiac and hepatic tissue after electric shock. The CK-MB, ALT, and AMS indexes were tested with serological methods. Moreover, the Bcl-2, Bax, and Hsp-60 expression levels were carefully measured. An electrical injury model was established by giving rats electric shocks at 110 V with alternating electric current. Blood samples from the rats were analyzed for the biochemical indexes. The degrees of pathological changes in the heart and liver were evaluated using IHC staining for Bcl-2, Bax, and Hsp-60. The levels of CK-MB in the electrical injury group rapidly peaked at 0.5 hours after the electric shock. Additionally, the levels of Bcl-2, Bax, and Hsp-60 in the cardiac and hepatic tissues changed regularly after the electrical injury and exhibited apparent differences from the levels in the control group. CK-MB, ALT, and AMS were altered regularly after electric shock, and these results provide significant information for clinical and medicolegal practice. This research has shed light on the assessment of electrical injury without obvious electrical burns. Furthermore, the findings obtained for Bcl-2/Bax and Hsp-60 can also facilitate pathological diagnosis and the identification of antemortem and postmortem electrical injury.
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影响因子：
2.7
作者：
Dokov, William
通讯作者：
Dokov, William
DOI：
10.1073/pnas.041619798
发表时间：
2001-03-13
影响因子：
11.1
作者：
Petros, AM;Medek, A;Fesik, SW
通讯作者：
Fesik, SW
影响因子：
1.5
作者：
Ghandour, Nagwa M.;Refaiy, Abeer E.;Omran, Ghada Ali
通讯作者：
Omran, Ghada Ali
影响因子：
4.4
作者：
Yang, RZ;Blaileanu, G;Gong, DW
通讯作者：
Gong, DW
影响因子：
7.4
作者：
Hollander, JM;Lin, KM;Dillmann, WH
通讯作者：
Dillmann, WH