Patterns of Genomic Variation in the Opportunistic Pathogen Candida glabrata Suggest the Existence of Mating and a Secondary Association with Humans.
Patterns of Genomic Variation in the Opportunistic Pathogen Candida glabrata Suggest the Existence of Mating and a Secondary Association with Humans.
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机会性病原体念珠菌中基因组变异的模式表明，交配的存在和与人类的二次关联。
DOI：
10.1016/j.cub.2017.11.027
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发表时间：
2018-01-08
期刊：
影响因子：
--
通讯作者：
Gabaldón T
中科院分区：
文献类型：
--
作者：
Carreté L;Ksiezopolska E;Pegueroles C;Gómez-Molero E;Saus E;Iraola-Guzmán S;Loska D;Bader O;Fairhead C;Gabaldón T
光滑念珠菌是一种机会性真菌病原体，是系统性念珠菌病的第二大常见病因。尽管它的属名，这种酵母是更密切相关的模式酵母酿酒酵母比其他念珠菌病原体，因此它感染人类的能力被认为是独立出现。此外，光滑隐翅虫具有经历性周期所需的所有基因，但由于缺乏有性生殖的直接证据而被认为是无性生物。为了重建这种病原体的最新进化并找到有性繁殖的足迹，我们评估了全球分布的33个光滑念珠菌分离株的基因组和表型变异。我们编目了广泛的拷贝数变异，特别是影响编码细胞壁相关蛋白质的基因，包括粘附素。光滑念珠菌中观察到的遗传变异水平显著高于白色念珠菌。这种变化被结构化成七个非常不同的分支，这表明最近的地理分散和大的分支内的基因组和表型差异。我们展示了令人信服的证据，最近的混合物之间的分化谱系和纯化选择交配基因，这提供了第一个证据，在这种酵母中存在一个活跃的性周期。总而言之，我们的数据表明，最近全球传播的先前遗传隔离的人口，并表明，人类只是一个次要的利基这种酵母。光滑念珠菌菌株可以聚集成遗传上高度不同的进化枝遗传结构表明先前分离的种群最近在全球传播光滑念珠菌中性别的存在得到了选择交配的基因组足迹的支持。型转换发生在光滑念珠菌自然种群中，但容易出错。对全球分布的新出现的真菌病原体念珠菌分离株的基因组分析表明，孤立的人群，并表明人类最有可能是这种酵母的次要利基。Carreté等人在这种所谓的“无性”病原体中找到重组和交配存在的证据。
Candida glabrata is an opportunistic fungal pathogen that ranks as the second most common cause of systemic candidiasis. Despite its genus name, this yeast is more closely related to the model yeast Saccharomyces cerevisiae than to other Candida pathogens, and hence its ability to infect humans is thought to have emerged independently. Moreover, C. glabrata has all the necessary genes to undergo a sexual cycle but is considered an asexual organism due to the lack of direct evidence of sexual reproduction. To reconstruct the recent evolution of this pathogen and find footprints of sexual reproduction, we assessed genomic and phenotypic variation across 33 globally distributed C. glabrata isolates. We cataloged extensive copy-number variation, which particularly affects genes encoding cell-wall-associated proteins, including adhesins. The observed level of genetic variation in C. glabrata is significantly higher than that found in Candida albicans. This variation is structured into seven deeply divergent clades, which show recent geographical dispersion and large within-clade genomic and phenotypic differences. We show compelling evidence of recent admixture between differentiated lineages and of purifying selection on mating genes, which provides the first evidence for the existence of an active sexual cycle in this yeast. Altogether, our data point to a recent global spread of previously genetically isolated populations and suggest that humans are only a secondary niche for this yeast. Candida glabrata strains can be clustered into highly genetically divergent clades Genetic structure suggests a recent global spread of previously isolated populations The existence of sex in C. glabrata is supported by genomic footprints of selection Mating-type switching occurs in C. glabrata natural populations but is error prone Genome analyses of globally distributed isolates of the emerging fungal pathogen Candida point to a recent global spread of previously isolated populations, and suggest that humans are most likely a secondary niche for this yeast. Carreté et al. find evidence for the existence of recombination and mating in this purported “asexual” pathogen.
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影响因子：
3.2
作者：
Gabaldón T;Carreté L
通讯作者：
Carreté L
影响因子：
64.8
作者：
Dujon, B;Sherman, D;Souciet, JL
通讯作者：
Souciet, JL
影响因子：
5.3
作者：
Andrulis, ED;Zappulla, DC;Sternglanz, R
通讯作者：
Sternglanz, R
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
使用orthomcl将蛋白质分配给Orthomcl-DB组或将蛋白质组群集分为新的直系同源组。
影响因子：
--
作者：
Fischer, Steve;Brunk, Brian P;Chen, Feng;Gao, Xin;Harb, Omar S;Iodice, John B;Shanmugam, Dhanasekaran;Roos, David S;Stoeckert, Christian J Jr
通讯作者：
Stoeckert, Christian J Jr