An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice.
An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice.
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DOI：
10.1186/1471-2164-8-175
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发表时间：
2007-06-18
期刊：
影响因子：
4.4
通讯作者：
de los Reyes BG
中科院分区：
文献类型：
--
作者：
Cheng C;Yun KY;Ressom HW;Mohanty B;Bajic VB;Jia Y;Yun SJ;de los Reyes BG
植物通过复杂的转录网络对低温做出反应。CBF/DREB调控的基因网络在拟南芥的低温适应过程中起重要作用。最近的证据还表明，CBF/DREB调节子不是CA所独有的，而是在寒冷不敏感（温带）和寒冷敏感（暖季）植物之间进化上保守的。本研究以籼稻和粳稻在低温敏感性上的巨大差异为模型，通过启动子结构和基因表达谱的整合分析，来鉴定其他调控簇。耐冷粳稻的转录组分析确定了121个“早期反应”基因的子集，这些基因在10°C的最初24小时内上调。在这一组中有四个转录因子，包括ROS-bZIP 1和另一个较大的亚组，其共同特征是在其启动子中具有as 1/ocs样元件。低温诱导ROS-bZIP 1基因先于as 1/ocs样元件基因的表达，在常温下外源H2 O2也能诱导这些基因的表达。协调的表达模式和类似的启动子结构之间的“早期反应”基因表明，它们属于一个潜在的调节子（ROS-bZIP -as 1/ocs调节模块），响应在低温胁迫期间ROS水平升高。候选基因的品种特异性表达特征表明，推定的调节子的活性和耐冷性的基因型变异之间呈正相关。在水稻中组装了一个由低温胁迫触发的ROS介导的调节子（ROS-bZIP -as 1/ocs）的假设模型。基于目前的研究结果，这似乎是独立的阿坝和CBF/DREB的调节子，它的激活有重要的贡献，在配置水稻幼苗对低温胁迫的快速反应。
Plants respond to low temperature through an intricately coordinated transcriptional network. The CBF/DREB-regulated network of genes has been shown to play a prominent role in freeze-tolerance of Arabidopsis through the process of cold acclimation (CA). Recent evidence also showed that the CBF/DREB regulon is not unique to CA but evolutionarily conserved between chilling-insensitive (temperate) and chilling-sensitive (warm-season) plants. In this study, the wide contrast in chilling sensitivity between indica and japonica rice was used as model to identify other regulatory clusters by integrative analysis of promoter architecture (ab initio) and gene expression profiles. Transcriptome analysis in chilling tolerant japonica rice identified a subset of 121 'early response' genes that were upregulated during the initial 24 hours at 10°C. Among this group were four transcription factors including ROS-bZIP1 and another larger sub-group with a common feature of having as1/ocs-like elements in their promoters. Cold-induction of ROS-bZIP1 preceded the induction of as1/ocs-like element-containing genes and they were also induced by exogenous H2O2 at ambient temperature. Coordinated expression patterns and similar promoter architectures among the 'early response' genes suggest that they belong to a potential regulon (ROS-bZIP – as1/ocs regulatory module) that responds to elevated levels of ROS during chilling stress. Cultivar-specific expression signatures of the candidate genes indicate a positive correlation between the activity of the putative regulon and genotypic variation in chilling tolerance. A hypothetical model of an ROS-mediated regulon (ROS-bZIP – as1/ocs) triggered by chilling stress was assembled in rice. Based on the current results, it appears that this regulon is independent of ABA and CBF/DREB, and that its activation has an important contribution in configuring the rapid responses of rice seedlings to chilling stress.
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影响因子：
6.9
作者：
Bolwell, GP;Bindschedler, LV;Minibayeva, F
通讯作者：
Minibayeva, F
影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
DOI：
10.1073/pnas.0307687100
发表时间：
2004-01-13
影响因子：
11.1
作者：
Dai, SH;Zhang, ZH;Beachy, RN
通讯作者：
Beachy, RN
DOI：
10.1073/pnas.0406069101
发表时间：
2004-10-19
影响因子：
11.1
作者：
Cook, D;Fowler, S;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
11.4
作者：
Delaunay, A;Isnard, AD;Toledano, MB
通讯作者：
Toledano, MB