Membrane glycoproteins involved in hepatocyte adhesion to collagen type I.
Membrane glycoproteins involved in hepatocyte adhesion to collagen type I.
复制标题
膜糖蛋白参与肝细胞与 I 型胶原蛋白的粘附。
DOI：
10.1016/0014-4827(88)90202-9
复制
发表时间：
1988
影响因子：
3.7
通讯作者：
Rubin,K
中科院分区：
文献类型：
--
作者：
Gullberg,D;Terracio,L;Rubin,K
Liver membrane glycoproteins with affinity for immobilized collagen type I were subjected to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electroelution of the separated proteins. Electroeluted glycoproteins with ability to neutralize the inhibitory effect of anti-CollCAM antibodies on hepatocyte adhesion to collagen were collected from several consecutive runs and used to raise a high titer antiserum, denoted anti-CollCAM II. IgG from this antiserum inhibited the attachment of hepatocytes to dishes coated with collagen type I, but not to fibronectin- or collagen type IV-coated dishes. When the antibodies were immobilized to Sepharose CL-4B they bound three sets of glycoproteins with apparentMr's of 105,000, 115,000, and 130,000 as analyzed by SDS-PAGE under nonreducing (NR) conditions. Upon reduction (R) the glycoproteins migrated with apparentMr's of 115,000, 130,000, and 160,000, respectively. TheMr105,000-115,000 (NR) glycoproteins effectively neutralized the inhibitory effect exerted by both anti-CollCAM and anti-CollCAM II antibodies, on hepatocyte spreading and attachment to collagen type I substrates. Peptide mapping suggested theMr160,000 (R) species to be different from theMr115,000 (R).
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成纤维细胞主要胶原结合蛋白的磷酸化和转化敏感性。
影响因子：
4.8
作者：
K. Nagata;K. Yamada
通讯作者：
K. Yamada
大鼠肝细胞的基质粘附：胶原蛋白基质的附着机制
影响因子：
64.5
作者：
K. Rubin;M. Höök;B. Öbrink;R. Timpl
通讯作者：
R. Timpl
影响因子：
2.2
作者：
LANDEGREN, U
通讯作者：
LANDEGREN, U
影响因子：
3.7
作者：
OCKLIND, C;ODIN, P;OBRINK, B
通讯作者：
OBRINK, B
影响因子：
2.7
作者：
BORG, TK;RUBIN, K;OBRINK, B
通讯作者：
OBRINK, B