Anti-leptin receptor antibody mimics the stimulation of lipolysis induced by leptin in isolated mouse fat pads.
Anti-leptin receptor antibody mimics the stimulation of lipolysis induced by leptin in isolated mouse fat pads.
复制标题
抗瘦素受体抗体模拟瘦素在离体小鼠脂肪垫中诱导的脂肪分解刺激。
作者：
N. Kawaji;A. Yoshida;T. Motoyashiki;T. Morita;H. Ueki
抗瘦素受体多克隆抗体（受体抗体），以及瘦素，以一种时间依赖性的方式刺激游离脂肪酸从分离的小鼠脂肪垫释放。孵育90 min后，在6 μ g/ml受体抗体和0.1 nM瘦素浓度下，脂肪溶解最大。受体抗体未显示出对瘦素诱导的脂肪分解刺激的任何附加效应，表明它们通过涉及瘦素受体的类似机制发挥作用。N-[2-（对溴肉桂胺）乙基]-5-异喹啉磺酰胺（H-89）、quin - 2- am、N-（6-氨基己基）-5-氯-1-萘磺酰胺（W-7）和硫酸新霉素（新霉素）均能有效抑制受体抗体和瘦素对脂肪分解的刺激。用受体抗体或瘦素对脂肪垫进行短期孵育，发现cAMP和肌醇1,4,5-三磷酸（IP3）的细胞含量短暂增加，其浓度与游离脂肪酸释放的浓度相似。Quin 2- am和W-7也抑制了cAMP含量的增加，提示Ca(2)+/钙调素依赖性过程可能参与了受体抗体和瘦素发挥作用的部分机制。通过对新霉素敏感的磷酸肌苷特异性磷脂酶C （PLC）增加细胞IP3含量似乎是启动细胞内事件的首要步骤。受体抗体和瘦素都可能通过细胞IP3含量的短暂增加以及cAMP的产生来刺激脂肪分解，从而导致cAMP依赖性蛋白激酶的激活。
An anti-leptin receptor polyclonal antibody (receptor antibody), as well as leptin, stimulated the release of free fatty acids from isolated mouse fat pads in a time-dependent manner. Following a 90-min incubation, maximal lipolysis was observed at 6 microg/ml receptor antibody and 0.1 nM leptin. The receptor antibody did not show any additive effect to the stimulation of lipolysis induced by leptin, suggesting that they exert their actions through a similar mechanism involving the leptin receptor. N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), quin 2-AM, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), and neomycin sulfate (neomycin) all potently inhibited the stimulation of lipolysis by the receptor antibody and leptin. Short-term incubation of the fat pads with the receptor antibody or leptin showed a transient increase in the cellular content of cAMP and myo-inositol 1,4,5-trisphosphate (IP3) in similar concentrations to the free fatty acid release. Quin 2-AM and W-7 also inhibited the increase in cAMP content, suggesting that a Ca(2)+/calmodulin-dependent process may be involved in a part of the mechanism in which the receptor antibody and leptin exert their effects. The increase in cellular IP3 content via phosphoinositide-specific phospholipase C (PLC) sensitive to neomycin appears to be a primary step to initiate intracellular events. Both the receptor antibody and leptin may stimulate the lipolysis through mechanisms involving a transient increase in the cellular IP3 content followed by cAMP production, which leads to the activation of cAMP-dependent protein kinase.
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DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Herrera,R;Rosen,OM
通讯作者：
Rosen,OM
影响因子：
15.9
作者：
Chen, NG;Swick, AG;Romsos, DR
通讯作者：
Romsos, DR
肌醇 1,4,5-三磷酸从分离的脂肪细胞内质网动员 Ca2，但不从质膜动员 Ca2。
DOI：
10.1042/bj2360037
发表时间：
1986
期刊：
The Biochemical journal
影响因子：
--
作者：
Delfert,DM;Hill,S;Pershadsingh,HA;Sherman,WR;McDonald,JM
通讯作者：
McDonald,JM
影响因子：
56.9
作者：
HALAAS, JL;GAJIWALA, KS;FRIEDMAN, JM
通讯作者：
FRIEDMAN, JM
模拟胰岛素作用而不激活酪氨酸激酶的胰岛素受体单克隆抗体。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hawley,DM;Maddux,BA;Patel,RG;Wong,KY;Mamula,PW;Firestone,GL;Brunetti,A;Verspohl,E;Goldfine,ID
通讯作者：
Goldfine,ID