Delphinidin, a dietary anthocyanidin in pigmented fruits and vegetables: a new weapon to blunt prostate cancer growth.
Delphinidin, a dietary anthocyanidin in pigmented fruits and vegetables: a new weapon to blunt prostate cancer growth.
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DOI：
10.4161/cc.7.21.6969
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发表时间：
2008-11-01
期刊：
影响因子：
--
通讯作者：
Mukhtar H
中科院分区：
文献类型：
--
作者：
Bin Hafeez B;Asim M;Siddiqui IA;Adhami VM;Murtaza I;Mukhtar H
在最近的一篇文章中，我们发现了花青素，一种花青素，可以诱导高转移性前列腺癌(PCa)PC3细胞的凋亡和细胞周期停滞。在这些研究的基础上，我们提供了更多的证据，证明Delphinidin诱导雄激素耐药的人PCa 22Rν1细胞的凋亡和细胞周期停滞，并且这些作用伴随着对NFκB的抑制。我们观察到Delphinidin处理22Rν1细胞导致剂量依赖的(I)G2/M期细胞周期停滞，(Ii)诱导细胞凋亡(III)和抑制NFκB信号转导。由于通用caspase抑制剂Z-VAD-FMK可显著逆转这一作用，因此，Delphinidin诱导的细胞凋亡是通过激活caspase来实现的。Delphinidin作用于细胞后，(I)IKKγ(NEMO)的磷酸化，(Ii)NFκB抑制蛋白IκBα的磷酸化，(Iii)在Ser536处的NFκB/p65和在Ser529处的NFκB/p50的磷酸化，(Iv)NFκB/p65核转位，以及(V)NFκBDNA结合活性的降低。综上所述，我们的研究结果表明，Delphinidin通过激活Caspase诱导雄激素非依赖性和雄激素非依赖性人PCa细胞的凋亡，并且这种作用可能是由于抑制了NFκB信号转导。我们建议将飞雀素开发为一种新型的抗前列腺癌药物。
In a recent publication, we have shown that delphinidin, an anthocyanidin induces apoptosis and cell cycle arrest in highly metastatic human prostate cancer (PCa) PC3 cells. Extending these studies, we provide additional evidence that delphinidin induces apoptosis and cell cycle arrest in androgen refractory human PCa 22Rν1 cells and that these effects are concomitant with inhibition of NFκB. We observed that delphinidin treatment to 22Rν1 cells resulted in a dose-dependent (i) G2/M phase cell cycle arrest, (ii) induction of apoptosis (iii) and inhibition of NFκB signaling. The induction of apoptosis by delphinidin was mediated via activation of caspases since a general caspase inhibitor Z-VAD-FMK significantly reversed this effect. Delphinidin treatment to cells resulted in a dose-dependent decrease in (i) phosphorylation of IKKγ (NEMO), (ii) phosphorylation of NFκB inhibitory protein IκBα, (iii) phosphorylation of NFκB/p65 at Ser536 and NFκB/p50 at Ser529, (iv) NFκB/p65 nuclear translocation, and (v) NFκB DNA binding activity. Taken together, our data show that delphinidin induces apoptosis of both androgen independent and androgen refractory human PCa cells via activation of caspases and in addition, this effect might be due to inhibition of NFκB signaling. We suggest that delphinidin could be developed as a novel agent against PCa.
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影响因子：
6.4
作者：
Afaq, F;Saleem, M;Mukhtar, H
通讯作者：
Mukhtar, H
影响因子：
4.3
作者：
Azevedo, Luciana;Alves de Lima, Patricia L.;Salvadori, Daisy M. F.
通讯作者：
Salvadori, Daisy M. F.
影响因子：
64.5
作者：
HOCKENBERY, DM;OLTVAI, ZN;KORSMEYER, SJ
通讯作者：
KORSMEYER, SJ
影响因子：
4.7
作者：
Lamy, Sylvie;Beaulieu, Edith;Beliveu, Richard
通讯作者：
Beliveu, Richard
影响因子：
8.8
作者：
Bucher, N.;Britten, C. D.
通讯作者：
Britten, C. D.