Migration of marrow stromal cells in response to sustained release of stromal-derived factor-1alpha from poly(lactide ethylene oxide fumarate) hydrogels.
Migration of marrow stromal cells in response to sustained release of stromal-derived factor-1alpha from poly(lactide ethylene oxide fumarate) hydrogels.
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DOI：
10.1016/j.ijpharm.2009.12.063
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发表时间：
2010-05-10
影响因子：
5.8
通讯作者：
Jabbari, Esmaiel
中科院分区：
文献类型：
--
作者：
He, Xuezhong;Ma, Junyu;Jabbari, Esmaiel
关键词：
基质衍生因子-1 α（Stromal derived factor-1 α，SDF-1α）是一种重要的趋化因子，在骨髓基质细胞（bone marrow stromal cell，BMS）归巢过程中起重要作用。然而，它在组织再生中的应用受到其相对较短的半衰期和细胞归巢到损伤部位的时间依赖性的限制。研究SDF-1α在可降解聚乳酸环氧乙烷富马酸酯（PLEOF）水凝胶中的释放特性，并探讨SDF-1α缓释对BMS细胞迁移的影响。合成了三种聚（L-丙交酯）（PLA）组分为6，9和24%重量的PLEOF水凝胶。在加入趋化因子后，聚合混合物交联以产生负载SDF-1α的PLEOF水凝胶。对凝胶的溶胶分数、吸水率、降解率、SDF-1α负载效率和释放动力学以及骨髓基质细胞（BMS）迁移率进行表征。具有6和9%PLA级分的更亲水的水凝胶具有显著的突释，随后通过扩散持续释放21天。具有24%PLA级分的更疏水的水凝胶具有不太明显的突释，并且在第1-9天之间通过扩散显示出缓慢但恒定的释放，然后在第9 - 18天通过扩散-降解显示出快速释放。21天后，从6%、9%和24%水凝胶释放的活性SDF-1α分数分别为34.3%、32.3%和35.8%。BMS细胞响应于时间释放的SDF-1α的迁移密切遵循水凝胶的蛋白质释放动力学。可生物降解的PLEOF水凝胶可能在组织工程应用中用作在愈合的增殖期持续释放SDF-1α的递送基质，用于招募祖细胞。
Stromal derived factor-1α (SDF-1α) is an important chemokine in stem cell trafficking and plays a critical role in the homing of bone marrow stromal (BMS) cells. However, its use in tissue regeneration is limited by its relatively short half-life and the time-dependent nature of cell homing to the site of injury. The objective of this work was to investigate the release characteristics of SDF-1α from degradable poly (lactide ethylene oxide fumarate) (PLEOF) hydrogels and to determine the effect of sustained release of SDF-1α on migration of BMS cells. Three PLEOF hydrogels with poly(L-lactide) (PLA) fractions of 6, 9, and 24% by weight were synthesized. After the addition of chemokine, the polymerizing mixture was crosslinked to produce SDF-1α loaded PLEOF hydrogels. The hydrogels were characterized with respect to sol fraction, water uptake, degradation, SDF-1α loading efficiency and release kinetics, and migration rate of bone marrow stromal (BMS) cells. The more hydrophilic hydrogels with 6 and 9% PLA fraction had a pronounced burst release followed by a period of sustained release by diffusion for 21 days. The more hydrophobic hydrogel with 24% PLA fraction had a less pronounced burst release and displayed a slow but constant release by diffusion between days 1–9 followed by a fast release by diffusion-degradation from days 9 to 18. The fraction of active SDF-1α released from 6, 9, and 24% hydrogels after 21 days was 34.3, 32.3, and 35.8%, respectively. The migration of BMS cells in response to time-released SDF-1α closely followed the protein release kinetics from the hydrogels. The biodegradable PLEOF hydrogel may potentially be useful as a delivery matrix for sustained release of SDF-1α in the proliferative phase of healing for recruitment of progenitor cells in tissue engineering applications.
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DOI：
10.1007/s10856-006-0020-2
发表时间：
2008-01-01
影响因子：
3.7
作者：
Jabbari, Esmaiel;He, Xuezhong
通讯作者：
He, Xuezhong
影响因子：
3.2
作者：
Gatti, R;Belletti, S;Gazzola, GC
通讯作者：
Gazzola, GC
DOI：
10.1016/j.molbrainres.2005.09.017
发表时间：
2005-12-07
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Ji, JF;Dheen, ST;Tay, SSW
通讯作者：
Tay, SSW
影响因子：
6
作者：
Jabbari, E;Nozari, S
通讯作者：
Nozari, S
影响因子：
5.2
作者：
Ji, JF;He, BP;Tay, SSW
通讯作者：
Tay, SSW