Identification of a potential FSH modulatory protein in human testis and seminal plasma.
Identification of a potential FSH modulatory protein in human testis and seminal plasma.
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鉴定人类睾丸和精浆中潜在的 FSH 调节蛋白。
DOI：
10.1002/j.1939-4640.1989.tb00125.x
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发表时间：
1989
影响因子：
--
通讯作者：
Cromie,WJ
中科院分区：
文献类型：
--
作者：
Sluss,PM;Schneyer,AL;Cockett,AT;Cromie,WJ
在雄性和雌性的性腺组织中，已经鉴定出多种能够抑制FSH与其受体体外结合的因子。由于它们作为促性腺激素作用的局部调节剂的潜在作用，对这些因子的兴趣已经被激发。本文报告的研究旨在确定人睾丸组织或精浆中是否存在与人FSH或从猪卵泡液中分离的“FSH样”蛋白具有抗原同源性的蛋白质。针对含有FSH受体结合抑制活性、FSH体外激动剂活性和人FSH抗血清识别的58，000 Mr蛋白的猪卵泡液组分产生多克隆抗体。抗猪卵泡液的抗血清和抗人FSH的抗血清用于探测来自人睾丸匀浆或精浆的蛋白质的Western印迹。在人睾丸提取物和精浆中鉴定出一种58，000 Mr的蛋白质。该蛋白似乎与人FSH和猪卵泡液中的58，000 Mr“FSH样”蛋白抗原相关。它似乎不是人FSH的代谢降解产物，因为该蛋白质大于FSH，在还原条件下不会解离成亚基，并且被不识别人FSH的FSH样蛋白的抗血清识别。这些数据确定了人睾丸和精浆中的一种58，000 Mr蛋白，可能代表睾丸中FSH作用的局部调节剂。
A variety of factors capable of inhibiting thein vitrobinding of FSH to its receptor have been identified in gonadal tissues from males and females. Interest in these factors has been stimulated because of their potential role as local modulators of gonadotropin action. Studies reported here were undertaken to determine if proteins having antigenic homologies with human FSH or an “FSH‐like” protein isolated from porcine follicular fluid were present in human testicular tissue or seminal plasma. Polyclonal antibodies were generated against fractions of porcine follicular fluid containing FSH receptor binding inhibitory activity, FSH agonist activityin vitro, and a 58,000 Mrprotein recognized by human FSH antiserum. Antiserum against this fraction of porcine follicular fluid and antiserum against human FSH were used to probe Western blots of proteins from human testis homogenates or seminal plasma. A 58,000 Mrprotein was identified in both human testis extract and seminal plasma. This protein appears to be related antigenically to both human FSH and the 58,000 Mr“FSH‐like” protein in porcine follicular fluid. It does not appear to be a metabolic degradatory product of human FSH since the protein is larger than FSH, does not dissociate into subunits under reducing conditions and is recognized by the antiserum to FSH‐like protein that does not recognize human FSH. These data identify a 58,000 Mrprotein in human testis and seminal plasma that may represent a local modulator of FSH action in the testis.
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DOI：
--
发表时间：
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