Recycling of epidermal growth factor-receptor complexes in A431 cells: identification of dual pathways.
Recycling of epidermal growth factor-receptor complexes in A431 cells: identification of dual pathways.
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DOI：
10.1083/jcb.112.1.55
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发表时间：
1991-01
期刊：
影响因子：
--
通讯作者：
Nikolsky N
中科院分区：
文献类型：
--
作者：
Sorkin A;Krolenko S;Kudrjavtceva N;Lazebnik J;Teslenko L;Soderquist AM;Nikolsky N
研究了表皮生长因子受体复合物（EGF-RC）在人表皮样癌A431细胞中的细胞内分选。研究发现，在37摄氏度内化后，EGF的再循环迅速发生。EGF的初始回收率在18 ℃时降低。一个重要的内源性EGF池在18摄氏度时不能再循环，但当细胞加热到37摄氏度时开始再循环。在37 ℃下，EGF从暴露于18 ℃温度块的细胞流出的相对速率（t1/2约20 min）比未暴露于温度块的细胞流出的速率（t1/2约5-7 min）慢。这些数据表明，在A431细胞中可能存在EGF的短期和长期循环。检查细胞内EGF-RC的解离和动力学的短期和长期的回收表明，EGF回收EGF-RC。此外，与125 I-EGF的光活化衍生物共价标记的EGF受体通过长时间途径以类似于125 I-EGF的速率再循环。由于EGF-RC降解也在18 ℃时被阻断，我们提出，溶酶体和长时间再循环途径的分选可能发生在一个高度温度敏感的步骤后，大概是在晚期内体中。
The intracellular sorting of EGF-receptor complexes (EGF-RC) has been studied in human epidermoid carcinoma A431 cells. Recycling of EGF was found to occur rapidly after internalization at 37 degrees C. The initial rate of EGF recycling was reduced at 18 degrees C. A significant pool of internalized EGF was incapable of recycling at 18 degrees C but began to recycle when cells were warmed to 37 degrees C. The relative rate of EGF outflow at 37 degrees C from cells exposed to an 18 degrees C temperature block was slower (t1/2 approximately 20 min) than the rate from cells not exposed to a temperature block (t1/2 approximately 5-7 min). These data suggest that there might be both short- and long-time cycles of EGF recycling in A431 cells. Examination of the intracellular EGF-RC dissociation and dynamics of short- and long-time recycling indicated that EGF recycled as EGF-RC. Moreover, EGF receptors that were covalently labeled with a photoactivatable derivative of 125I-EGF recycled via the long-time pathway at a rate similar to that of 125I-EGF. Since EGF-RC degradation was also blocked at 18 degrees C, we propose that sorting to the lysosomal and long-time recycling pathway may occur after a highly temperature-sensitive step, presumably in the late endosomes.
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影响因子：
7.8
作者：
MILLER, K;BEARDMORE, J;HOPKINS, CR
通讯作者：
HOPKINS, CR
影响因子：
3.9
作者：
BEARDMORE, J;HOPKINS, CR
通讯作者：
HOPKINS, CR
DOI：
10.1073/pnas.81.8.2384
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BEGUINOT, L;LYALL, RM;PASTAN, I
通讯作者：
PASTAN, I
影响因子：
64.5
作者：
GEUZE, HJ;SLOT, JW;SCHWARTZ, AL
通讯作者：
SCHWARTZ, AL
影响因子：
64.5
作者：
HONEGGER, AM;DULL, TJ;SCHLESSINGER, J
通讯作者：
SCHLESSINGER, J