Debiased ambient vibrations optical coherence elastography to profile cell, organoid and tissue mechanical properties.
Debiased ambient vibrations optical coherence elastography to profile cell, organoid and tissue mechanical properties.
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DOI：
10.1038/s42003-023-04788-0
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发表时间：
2023-05-18
影响因子：
5.9
通讯作者：
Bagnaninchi, Pierre O.
中科院分区：
文献类型：
--
作者：
Mason, Jonathan H.;Luo, Lu;Reinwald, Yvonne;Taffetani, Matteo;Hallas-Potts, Amelia;Herrington, C. Simon;Srsen, Vlastimil;Lin, Chih-Jen;Barroso, Ines A.;Zhang, Zhihua;Zhang, Zhibing;Ghag, Anita K.;Yang, Ying;Waters, Sarah;El Haj, Alicia J.;Bagnaninchi, Pierre O.
力学环境在定义组织功能、发育和生长中的作用已被证明是基本的。在多个尺度的组织基质的刚度变化的评估主要依赖于侵入性的，往往是专业的设备，如AFM或机械测试设备不适合细胞培养workflow.In本文中，我们已经开发出一种无偏的被动光学相干弹性成像方法，利用环境振动的样品，使实时非侵入性定量分析的细胞和组织。我们展示了一个强大的方法，通过积极补偿散射相关的噪声偏差和减少方差，使光学散射和机械性能。该方法检索基础事实的效率在计算机和体外进行了验证，并举例说明了关键应用，如骨和软骨球体的时程机械分析，组织工程癌症模型，组织修复模型和单细胞。我们的方法是很容易实现的任何商业光学相干断层扫描系统没有任何硬件修改，从而提供了一个突破，在线组织力学评估的空间机械性能的类器官，软组织和组织工程。描述了一种用于评估类器官、软组织和组织工程的空间机械特性的方法。
The role of the mechanical environment in defining tissue function, development and growth has been shown to be fundamental. Assessment of the changes in stiffness of tissue matrices at multiple scales has relied mostly on invasive and often specialist equipment such as AFM or mechanical testing devices poorly suited to the cell culture workflow.In this paper, we have developed a unbiased passive optical coherence elastography method, exploiting ambient vibrations in the sample that enables real-time noninvasive quantitative profiling of cells and tissues. We demonstrate a robust method that decouples optical scattering and mechanical properties by actively compensating for scattering associated noise bias and reducing variance. The efficiency for the method to retrieve ground truth is validated in silico and in vitro, and exemplified for key applications such as time course mechanical profiling of bone and cartilage spheroids, tissue engineering cancer models, tissue repair models and single cell. Our method is readily implementable with any commercial optical coherence tomography system without any hardware modifications, and thus offers a breakthrough in on-line tissue mechanical assessment of spatial mechanical properties for organoids, soft tissues and tissue engineering. A method is described for assessing spatial mechanical properties for organoids, soft tissues and tissue engineering.
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影响因子：
4.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
EHMAN, RL