Lipid Landscape of the Human Retina and Supporting Tissues Revealed by High-Resolution Imaging Mass Spectrometry.
Lipid Landscape of the Human Retina and Supporting Tissues Revealed by High-Resolution Imaging Mass Spectrometry.
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DOI：
10.1021/jasms.0c00119
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发表时间：
2020-12-02
影响因子：
3.2
通讯作者：
Schey KL
中科院分区：
文献类型：
--
作者：
Anderson DMG;Messinger JD;Patterson NH;Rivera ES;Kotnala A;Spraggins JM;Caprioli RM;Curcio CA;Schey KL
人类视网膜在从星光到阳光的光水平下提供视觉。其支持组织调节血浆递送的亲脂性视觉必需品，包括类维生素A。黄斑是一种解剖学上的特化，用于高敏锐度和彩色视觉，也容易受到流行的致盲疾病的影响。视网膜的精致结构包括许多水平排列的细胞类型，产生结构上不同的细胞，突触和血管层，在组织学和诊断临床成像中可见。MALDI成像质谱（IMS）现在能够将低微米空间分辨率与高水平的化学特异性结合起来。在这项研究中，一个多模态成像方法加强了准确的多图像配准被用来定位脂质在人类视网膜组织在层，细胞和亚细胞水平。多模态成像结果表明，在单细胞类型之间和内部的脂质种类的分布和丰度的差异。值得注意的是黄斑特定层内信号的不同定位。例如，磷脂酰乙醇胺和磷脂酰肌醇脂质定位于中央RPE细胞，而特定的缩醛磷脂脂质定位于中央凹周围RPE和Henle纤维层的细胞。光感受器的亚细胞区室由外核层中的PE（20：0_22：5）、外节和内节中的PE（18：0_22：6）以及富含维生素A的内节中的心磷脂CL（70：5）区分。几种脂质，不同的是一个单一的双键，中央凹和神经节细胞和内核层之间有显着不同的分布。在这项研究中启动的脂质图谱可以作为未来检查病变组织的参考数据库。
The human retina provides vision at light levels ranging from starlight to sunlight. Its supporting tissues regulate plasma-delivered lipophilic essentials for vision, including retinoids. The macula is an anatomic specialization for high-acuity and color vision that is also vulnerable to prevalent blinding diseases. The retina’s exquisite architecture comprises numerous cell types that are aligned horizontally, yielding structurally distinct cell, synaptic, and vascular layers that are visible in histology and in diagnostic clinical imaging. MALDI imaging mass spectrometry (IMS) is now capable of uniting low micrometer spatial resolution with high levels of chemical specificity. In this study, a multimodal imaging approach fortified with accurate multi-image registration was used to localize lipids in human retina tissue at laminar, cellular, and subcellular levels. Multimodal imaging results indicate differences in distributions and abundances of lipid species across and within single cell types. Of note are distinct localizations of signals within specific layers of the macula. For example, phosphatidylethanolamine and phosphatidylinositol lipids were localized to central RPE cells, whereas specific plasmalogen lipids were localized to cells of the perifoveal RPE and Henle fiber layer. Subcellular compartments of photoreceptors were distinguished by PE(20:0_22:5) in the outer nuclear layer, PE(18:0_22:6) in outer and inner segments, and cardiolipin CL(70:5) in the mitochondria-rich inner segments. Several lipids, differing by a single double bond, have markedly different distributions between the central fovea and the ganglion cell and inner nuclear layers. A lipid atlas, initiated in this study, can serve as a reference database for future examination of diseased tissues.
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影响因子：
5.1
作者：
Chen, Ying;Jester, James V.;Vasiliou, Vasilis
通讯作者：
Vasiliou, Vasilis
影响因子：
4.5
作者：
Messias MCF;Mecatti GC;Priolli DG;de Oliveira Carvalho P
通讯作者：
de Oliveira Carvalho P
影响因子：
4.4
作者：
Grey AC;Schey KL
通讯作者：
Schey KL
影响因子：
4.6
作者：
Anderson DMG;Ablonczy Z;Koutalos Y;Hanneken AM;Spraggins JM;Calcutt MW;Crouch RK;Caprioli RM;Schey KL
通讯作者：
Schey KL
影响因子：
4.8
作者：
Harkewicz, Richard;Du, Hongjun;Zhang, Kang
通讯作者：
Zhang, Kang