MicroRNA profiles in allograft tissues and paired urines associate with chronic allograft dysfunction with IF/TA.
MicroRNA profiles in allograft tissues and paired urines associate with chronic allograft dysfunction with IF/TA.
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同种异体移植组织中的microRNA谱和尿液配对与慢性同种异体功能障碍与IF/TA相关的。
DOI：
10.1111/j.1600-6143.2011.03666.x
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Mas V
中科院分区：
文献类型：
--
作者：
Scian MJ;Maluf DG;David KG;Archer KJ;Suh JL;Wolen AR;Mba MU;Massey HD;King AL;Gehr T;Cotterell A;Posner M;Mas V
Despite the advances in immunosuppression, renal allograft attrition over time remains unabated due to chronic allograft dysfunction (CAD) with interstitial fibrosis (IF) and tubular atrophy (TA). We aimed to evaluate microRNA (miRNA) signatures in CAD with IF/TA and appraise correlation with paired urine samples and potential utility in prospective evaluation of graft function. MicroRNA signatures were established between CAD with IF/TA vs. normal allografts by microarray. Validation of the microarray results and prospective evaluation of urine samples was performed using RT-qPCR. Fifty-six miRNAs were identified in samples with CAD-IF/TA. Five miRNAs were selected for further validation based on: array fold change, p-value and in silico predicted mRNA targets. We confirmed the differential expression of these 5 miRNAs by RT-qPCR using an independent set of samples. Differential expression was detected for miR-142-3p, miR-204, miR-107, and miR-211 (P<0.001) and miR-32 (p<0.05). Furthermore, differential expression of miR-142-3p (p<0.01), miR-204 (p<0.01) and miR-211 (p<0.05) was also observed between patient groups in urine samples. A characteristic miRNA signature for IF/TA that correlates with paired urine samples was identified. These results support the potential use of miRNAs as non-invasive markers of IF/TA and for monitoring graft function.
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