Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice.
Identifying metabolic pathways for production of extracellular polymeric substances by the diatom Fragilariopsis cylindrus inhabiting sea ice.
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DOI：
10.1038/s41396-017-0039-z
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Underwood GJC
中科院分区：
文献类型：
--
作者：
Aslam SN;Strauss J;Thomas DN;Mock T;Underwood GJC
硅藻是海冰中重要的初级生产者，海冰是一种短暂的栖息地，具有陡峭的温度和盐度垂直梯度，是冰基质环境的特征。为了科普多变和具有挑战性的条件，海冰硅藻产生富含多糖的胞外聚合物（EPS），其在粘附、细胞保护、配体结合和作为有机碳源方面发挥重要作用。南极威德尔海海冰和南极东部海冰的EPS浓度和化学组成随温度和盐度梯度的变化存在显著差异。为了重建硅藻产生EPS的第一条代谢途径，我们将一种关键的双极硅藻物种柱脆藻暴露于模拟海冰形成中。在不同的EPS生产条件下的转录组分析确定了大量的基因和不同的等位基因。它们在模拟海冰形成下的复杂差异表达模式与细胞的生理和生化特性以及海冰EPS特性的现场测量一致。因此，EPS途径的分子复杂性表明F.圆柱藻需要科普高度多变和极端的海冰栖息地的挑战性条件。
Diatoms are significant primary producers in sea ice, an ephemeral habitat with steep vertical gradients of temperature and salinity characterizing the ice matrix environment. To cope with the variable and challenging conditions, sea ice diatoms produce polysaccharide-rich extracellular polymeric substances (EPS) that play important roles in adhesion, cell protection, ligand binding and as organic carbon sources. Significant differences in EPS concentrations and chemical composition corresponding to temperature and salinity gradients were present in sea ice from the Weddell Sea and Eastern Antarctic regions of the Southern Ocean. To reconstruct the first metabolic pathway for EPS production in diatoms, we exposed Fragilariopsis cylindrus, a key bi-polar diatom species, to simulated sea ice formation. Transcriptome profiling under varying conditions of EPS production identified a significant number of genes and divergent alleles. Their complex differential expression patterns under simulated sea ice formation was aligned with physiological and biochemical properties of the cells, and with field measurements of sea ice EPS characteristics. Thus, the molecular complexity of the EPS pathway suggests metabolic plasticity in F. cylindrus is required to cope with the challenging conditions of the highly variable and extreme sea ice habitat.
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影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
4.2
作者：
Ewert M;Deming JW
通讯作者：
Deming JW
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
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通讯作者：
HOCHBERG, Y
影响因子：
5.1
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影响因子：
1.6
作者：
Herborg, LM;Thomas, DN;Dieckmann, GS
通讯作者：
Dieckmann, GS