Chemotaxis and Shorter O-Antigen Chain Length Contribute to the Strong Desiccation Tolerance of a Food-Isolated Cronobacter sakazakii Strain.
Chemotaxis and Shorter O-Antigen Chain Length Contribute to the Strong Desiccation Tolerance of a Food-Isolated Cronobacter sakazakii Strain.
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趋化性和较短的 O 抗原链长度有助于食品分离的坂崎克罗诺杆菌菌株的强干燥耐受性
DOI：
10.3389/fmicb.2021.779538
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发表时间：
2021
影响因子：
5.2
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Qian C;Huang M;Du Y;Song J;Mu H;Wei Y;Zhang S;Yin Z;Yuan C;Liu B;Liu B
阪崎克罗诺杆菌是一种机会致病菌，婴儿致死率高达80%。干燥耐受性确保其在婴儿配方粉（PIF）中存活，并有助于增加对新生儿的暴露，导致新生儿脑膜炎、败血症和坏死性小肠结肠炎。从食物分离的阪崎C. G4023菌株比阪崎C. ATCC 29544菌株具有更强的耐干燥性。考虑到已证实的G4023致病性，它可能对婴儿构成很大威胁。转录组学和蛋白质组学研究为G4023的干燥适应机制提供了新的见解。这些组学的综合分析表明，发现331个基因在转录和蛋白质水平上都受到调节（分别为≥2.0倍和≥1.5倍）。趋化系统编码基因cheA和cheW的缺失导致短期和长期干燥的耐受性下降。o抗原链长度的缩短对生物膜的形成和干燥耐受性有短期而非长期的促进作用。此外，在干燥的G4023中，鞭毛的生物合成、精氨酸及其运输系统、铁/硫簇也受到了调节。更好地了解G4023的干燥适应机制，可以指导PIF或其他食物的生产和保存操作，从而降低G4023的存活率，降低其对婴儿的暴露。
Cronobacter sakazakii is an opportunistic pathogen causing a lethality rate as high as 80% in infants. Desiccation tolerance ensures its survival in powdered infant formula (PIF) and contributes to the increased exposure to neonates, resulting in neonatal meningitis, septicemia, and necrotizing enterocolitis. This study showed that a food-isolated C. sakazakii G4023 strain exhibited a stronger desiccation tolerance than C. sakazakii ATCC 29544 strain. Considering the proven pathogenicity of G4023, it could be a big threat to infants. Transcriptome and proteome were performed to provide new insights into the desiccation adaptation mechanisms of G4023. Integrated analyses of these omics suggested that 331 genes were found regulated at both transcriptional and protein levels (≥2.0- and ≥1.5-fold, respectively). Deletion of chemotaxis system encoded genes cheA and cheW resulted in decreased tolerance in both short- and long-term desiccation. Reduced O-antigen chain length contributed to the biofilm formation and desiccation tolerance in the short term rather than the long term. In addition, biosynthesis of flagella, arginine and its transport system, and Fe/S cluster were also observed regulated in desiccated G4023. A better understanding of desiccation adaptation mechanisms of G4023 could in turn guide the operations during production and preservation of PIF or other food to reduce survival odds of G4023 and lower its exposure to get to infants.
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影响因子：
4.5
作者：
Ghazalpour A;Bennett B;Petyuk VA;Orozco L;Hagopian R;Mungrue IN;Farber CR;Sinsheimer J;Kang HM;Furlotte N;Park CC;Wen PZ;Brewer H;Weitz K;Camp DG 2nd;Pan C;Yordanova R;Neuhaus I;Tilford C;Siemers N;Gargalovic P;Eskin E;Kirchgessner T;Smith DJ;Smith RD;Lusis AJ
通讯作者：
Lusis AJ
DOI：
10.1099/00207713-30-3-569
发表时间：
1980-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
FARMER, JJ;ASBURY, MA;BRENNER, DJ
通讯作者：
BRENNER, DJ
影响因子：
8.1
作者：
Hu, Shuangfang;Yu, Yigang;Wu, Hui
通讯作者：
Wu, Hui
影响因子：
7
作者：
Han, Chia-Li;Chien, Chih-Wei;Chen, Yu-Ju
通讯作者：
Chen, Yu-Ju
影响因子：
7
作者：
Chen, GA;Gharib, TG;Beer, DG
通讯作者：
Beer, DG