Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology
Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology
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活性依赖性玻璃细胞力学Ⅰ：用活性微流变学测量机械性能
DOI：
10.1016/j.bpj.2023.04.011
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发表时间：
2023
影响因子：
3.4
通讯作者：
Mizuno Daisuke
中科院分区：
文献类型：
--
作者：
Ebata Hiroyuki;Umeda Katsuhiro;Nishizawa Kenji;Nagao Wataru;Inokuchi Shono;Sugino Yujiro;Miyamoto Takafumi;Mizuno Daisuke
采用反馈跟踪技术，通过施加光捕获力到剧烈波动的示踪剂珠上，在活细胞中进行主动微流变学。在上皮样融合单层中的HeLa细胞中测量复数剪切模量G（ω）=G′（ω）-IG″（ω）。我们发现在很宽的频率范围内（1Hz << 10 kHz）。肌动蛋白破坏和细胞周期从G1期到S期和G2期的进展对活细胞的影响有限。另一方面，被发现是依赖于细胞代谢; ATP耗竭的细胞表现出增加的弹性模量在低频率下，引起一个恒定的平台，这样。ATP耗尽细胞的平台和额外的频率依赖性与典型的胶体堵塞的流变学反应一致。另一方面，普通代谢活跃的细胞中的平台消失了，这意味着活细胞使其内部状态流化，从而接近临界堵塞点。
Active microrheology was conducted in living cells by applying an optical-trapping force to vigorously fluctuating tracer beads with feedback-tracking technology. The complex shear modulus G(ω)=G′(ω)−iG″(ω) was measured in HeLa cells in an epithelial-like confluent monolayer. We found thatover a wide range of frequencies (1 Hz << 10 kHz). Actin disruption and cell-cycle progression from G1 to S and G2 phases only had a limited effect onin living cells. On the other hand,was found to be dependent on cell metabolism; ATP-depleted cells showed an increased elastic modulusat low frequencies, giving rise to a constant plateau such that. Both the plateau and the additional frequency dependencyof ATP-depleted cells are consistent with a rheological response typical of colloidal jamming. On the other hand, the plateaudisappeared in ordinary metabolically active cells, implying that living cells fluidize their internal states such that they approach the critical jamming point.
微机械测量中的信号和噪声。
影响因子：
--
作者：
F. Gittes;C. Schmidt
通讯作者：
C. Schmidt
影响因子：
2.4
作者：
Hough, LA;Ou-Yang, HD
通讯作者：
Ou-Yang, HD
影响因子：
3.4
作者：
Liang, H;Vu, KT;Berns, MW
通讯作者：
Berns, MW