Retinoid X receptor modulates olfactory attraction through Gα signaling in the migratory locusts.
Retinoid X receptor modulates olfactory attraction through Gα signaling in the migratory locusts.
复制标题
类维生素A X 受体通过Gα 信号调节飞蝗的嗅觉吸引力。
DOI：
10.1016/j.ibmb.2019.103265
复制
发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
Liu JP
中科院分区：
文献类型：
--
作者：
Ma ZY;Liu JP
动物为了生存和适应而聚集在一起相互交流。在集群过程中，独居蝗虫的嗅觉表现出从排斥到吸引的转变。然而，这种转变背后的分子机制却鲜为人知。在这项研究中，我们探讨了蝗虫聚集过程中差异表达的转录本（DET），并确定了一个功能类的一般代谢包括最大数量的DET在所有分析的基因类。在一般代谢的这类功能中，氧化还原酶介导维生素A和其他碳水化合物代谢产物合成维甲酸（RA）。视黄酸脱氢酶1（raldh1）和类维生素A X受体（rxr）是RA合成和信号传导的两个关键基因，其表达水平在拥挤4 h期间上调。通过RNA干扰（RNAi）在大脑中敲低raldh1和rxr导致嗅觉吸引力的丧失。此外，通过RNA干扰抑制RXR导致Gna 14的表达下调，Gna 14是Gα亚家族的成员，在G蛋白偶联受体（GPCR）途径中传递信号。通过RNAi技术阻断RXR信号通路和Gna14抑制多巴胺受体1（DopR1）和章鱼胺受体α1（OctαR1）调节嗅觉吸引的功能。RXR信号是DopR1和OctαR1介导嗅觉吸引所必需的。这项研究表明，RXR信号通过Gα信号介导吸引，并证实了核受体RXR和膜受体GPCRs在调节嗅觉吸引中的新联系。
Animals communicate with each other in aggregating for survival and adaptation. Solitary locusts show an olfactory transition from repulsion to attraction in aggregation. However, the molecular mechanism underlying this transition is less well known. In this study, we explored differentially expressed transcripts (DETs) during locust aggregation and identified that a functional class of general metabolism encompassed the largest number of DETs among all analyzed gene classes. Within this functional class of general metabolism, oxidoreductase mediates synthesis of retinoic acid (RA) from vitamin A and other metabolites derived from carbohydrates. The expression levels ofretinaldehydehydroxylase1(raldh1) and retinoid X receptor(rxr), which are two crucial genes for RA synthesis and signaling, were upregulated during 4 h of crowding. Knockdown ofraldh1andrxrby RNA interference (RNAi) in the brains resulted in the loss of olfactory attraction. Moreover, inhibition of RXR by RNAi resulted in downregulated expression ofGna14, a member of the Gα subfamily that transduces signals in G protein-coupled receptor (GPCR) pathways. Abrogating RXR signaling andGna14 by RNAi knockdown inhibited the function of dopamine receptor 1 (DopR1) and octopamine receptor α1 (OctαR1) in modulating olfactory attraction. RXR signaling is essential for DopR1 and OctαR1 to mediate olfactory attraction. This study showed that RXR signaling mediates attraction by Gα signaling and confirmed a novel link between nuclear receptor RXR and the membrane receptor GPCRs in modulating olfactory attraction.
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