Projection Structures of Biological Cells and Organelles
Projection Structures of Biological Cells and Organelles
复制标题
生物细胞和细胞器的投影结构
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
M. Nakasako
中科院分区：
文献类型：
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作者：
M. Nakasako
X射线衍射成像（XDI）的衍射装置和样品制备方法使我们能够在66-80 K收集冷冻水合非结晶生物颗粒（如细胞和细胞器）的衍射图案。从高品质的衍射图案，相位恢复（PR）计算重建样品粒子的电子密度图投影沿着入射X射线的方向。在这里，应用XDI的细胞和细胞成分的结构研究。同步加速器XDI的主题包括菠菜和小型真核生物Cyanidioschyzonmerolae叶绿体的结构分析。在利用X射线自由电子激光（XFEL）脉冲进行的XDI实验中，对蓝藻细胞和C。梅若莱进行了调查。此外，由于XFEL-XDI实验可以在短时间内提供大量的衍射图案，因此可以构建具有统计学意义的目标颗粒的尺寸分布。作为例子，氧化亚铜颗粒，蓝藻细胞，淀粉样蛋白聚集体的大小分布。
The diffraction apparatus for X-ray diffraction imaging (XDI) and the specimen preparation methods allow us to collect diffraction patterns from frozen-hydrated noncrystalline biological particles, such as cells and organelles, at 66–80 K. From high-quality diffraction patterns, phase retrieval (PR) calculations reconstruct electron density maps of specimen particles projected along the direction of the incident X-rays. Here, the application of XDI to structural studies of cells and cellular components is presented. Topics in synchrotron XDI include structural analyses of the chloroplasts of spinach and the small eukaryote Cyanidioschyzon merolae . In XDI experiments using X-ray free electron laser (XFEL) pulses, the structure of cyanobacteria cells and the chloroplasts of C. merolae are investigated. In addition, because XFEL-XDI experiments can provide a large number of diffraction patterns within a short time, the size distribution of targeted particles can be constructed with statistical significance. As examples, the size distributions of cuprous oxide particles, cyanobacteria cells, and amyloid aggregates are presented.
影响因子：
5.6
作者：
Lashuel, HA;Petre, BM;Lansbury, PT
通讯作者：
Lansbury, PT
影响因子：
64.8
作者：
Seibert, M. Marvin;Ekeberg, Tomas;Maia, Filipe R. N. C.;Svenda, Martin;Andreasson, Jakob;Joensson, Olof;Odic, Dusko;Iwan, Bianca;Rocker, Andrea;Westphal, Daniel;Hantke, Max;DePonte, Daniel P.;Barty, Anton;Schulz, Joachim;Gumprecht, Lars;Coppola, Nicola;Aquila, Andrew;Liang, Mengning;White, Thomas A.;Martin, Andrew;Caleman, Carl;Stern, Stephan;Abergel, Chantal;Seltzer, Virginie;Claverie, Jean-Michel;Bostedt, Christoph;Bozek, John D.;Boutet, Sebastien;Miahnahri, A. Alan;Messerschmidt, Marc;Krzywinski, Jacek;Williams, Garth;Hodgson, Keith O.;Bogan, Michael J.;Hampton, Christina Y.;Sierra, Raymond G.;Starodub, Dmitri;Andersson, Inger;Bajt, Sasa;Barthelmess, Miriam;Spence, John C. H.;Fromme, Petra;Weierstall, Uwe;Kirian, Richard;Hunter, Mark;Doak, R. Bruce;Marchesini, Stefano;Hau-Riege, Stefan P.;Frank, Matthias;Shoeman, Robert L.;Lomb, Lukas;Epp, Sascha W.;Hartmann, Robert;Rolles, Daniel;Rudenko, Artem;Schmidt, Carlo;Foucar, Lutz;Kimmel, Nils;Holl, Peter;Rudek, Benedikt;Erk, Benjamin;Hoemke, Andre;Reich, Christian;Pietschner, Daniel;Weidenspointner, Georg;Strueder, Lothar;Hauser, Guenter;Gorke, Hubert;Ullrich, Joachim;Schlichting, Ilme;Herrmann, Sven;Schaller, Gerhard;Schopper, Florian;Soltau, Heike;Kuehnel, Kai-Uwe;Andritschke, Robert;Schroeter, Claus-Dieter;Krasniqi, Faton;Bott, Mario;Schorb, Sebastian;Rupp, Daniela;Adolph, Marcus;Gorkhover, Tais;Hirsemann, Helmut;Potdevin, Guillaume;Graafsma, Heinz;Nilsson, Bjoern;Chapman, Henry N.;Hajdu, Janos
通讯作者：
Hajdu, Janos