Single mimivirus particles intercepted and imaged with an X-ray laser.
Single mimivirus particles intercepted and imaged with an X-ray laser.
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DOI：
10.1038/nature09748
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发表时间：
2011-02-03
期刊：
影响因子：
64.8
通讯作者：
Hajdu, Janos
中科院分区：
文献类型：
--
作者：
Seibert, M. Marvin;Ekeberg, Tomas;Maia, Filipe R. N. C.;Svenda, Martin;Andreasson, Jakob;Joensson, Olof;Odic, Dusko;Iwan, Bianca;Rocker, Andrea;Westphal, Daniel;Hantke, Max;DePonte, Daniel P.;Barty, Anton;Schulz, Joachim;Gumprecht, Lars;Coppola, Nicola;Aquila, Andrew;Liang, Mengning;White, Thomas A.;Martin, Andrew;Caleman, Carl;Stern, Stephan;Abergel, Chantal;Seltzer, Virginie;Claverie, Jean-Michel;Bostedt, Christoph;Bozek, John D.;Boutet, Sebastien;Miahnahri, A. Alan;Messerschmidt, Marc;Krzywinski, Jacek;Williams, Garth;Hodgson, Keith O.;Bogan, Michael J.;Hampton, Christina Y.;Sierra, Raymond G.;Starodub, Dmitri;Andersson, Inger;Bajt, Sasa;Barthelmess, Miriam;Spence, John C. H.;Fromme, Petra;Weierstall, Uwe;Kirian, Richard;Hunter, Mark;Doak, R. Bruce;Marchesini, Stefano;Hau-Riege, Stefan P.;Frank, Matthias;Shoeman, Robert L.;Lomb, Lukas;Epp, Sascha W.;Hartmann, Robert;Rolles, Daniel;Rudenko, Artem;Schmidt, Carlo;Foucar, Lutz;Kimmel, Nils;Holl, Peter;Rudek, Benedikt;Erk, Benjamin;Hoemke, Andre;Reich, Christian;Pietschner, Daniel;Weidenspointner, Georg;Strueder, Lothar;Hauser, Guenter;Gorke, Hubert;Ullrich, Joachim;Schlichting, Ilme;Herrmann, Sven;Schaller, Gerhard;Schopper, Florian;Soltau, Heike;Kuehnel, Kai-Uwe;Andritschke, Robert;Schroeter, Claus-Dieter;Krasniqi, Faton;Bott, Mario;Schorb, Sebastian;Rupp, Daniela;Adolph, Marcus;Gorkhover, Tais;Hirsemann, Helmut;Potdevin, Guillaume;Graafsma, Heinz;Nilsson, Bjoern;Chapman, Henry N.;Hajdu, Janos
X射线激光为理解极端条件下生物系统、复杂材料和物质的结构提供了新的能力。非常短且极其明亮的相干X射线脉冲可以用来绕过关键的损伤过程，并在样品爆炸并转变为等离子体之前从大分子、病毒或细胞获得单一的衍射图。非晶态物体的连续衍射图允许过采样和直接相位恢复。在这里，我们展示了从非晶态生物样品，单个拟态病毒粒子，注入硬X射线自由电子激光器的脉冲光束，直线加速器相干光源的单个X射线脉冲可以获得高质量的衍射数据。计算表明，脉冲离开样本后，脉冲沉积到病毒中的能量将粒子加热到超过10万K。重建的出射波前(图像)在一次曝光中产生了32 nm的全周期分辨率，并且没有显示出可测量的损害。重建表明病毒粒子内部的致密物质排列不均匀。我们预计，在这样的实验中，随着更短、更明亮的光子脉冲聚焦到更小的区域，将实现显著更高的分辨率。这种实验中的分辨率可以进一步扩展到有多个相同副本的样品。
X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.
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影响因子：
2.1
作者：
Luke, DR
通讯作者：
Luke, DR
影响因子：
56.9
作者：
Perkins, TT;Smith, DE;Chu, S
通讯作者：
Chu, S
影响因子：
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作者：
Emma, P.;Akre, R.;Galayda, J.
通讯作者：
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影响因子：
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作者：
Song, Changyong;Jiang, Huaidong;Miao, Jianwei
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DOI：
10.1088/0953-4075/43/19/194015
发表时间：
2010-10-14
影响因子：
1.6
作者：
Seibert, M. Marvin;Boutet, Sebastien;Hajdu, Janos
通讯作者：
Hajdu, Janos