Quantifying bursting neuron activity from calcium signals using blind deconvolution.
Quantifying bursting neuron activity from calcium signals using blind deconvolution.
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DOI：
10.1016/j.jneumeth.2013.05.007
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发表时间：
2013-09-15
影响因子：
3
通讯作者：
Principe, Jose C.
中科院分区：
文献类型：
--
作者：
Park, In Jun;Bobkov, Yuriy V.;Ache, Barry W.;Principe, Jose C.
关键词：
钙成像技术的进步使得研究单个神经元和神经元集合的动态活动成为可能。然而，挑战，如未知的非线性峰-钙关系，噪声，钙信号的时间分辨率相对于峰产生的时间尺度通常相对较低，限制了从钙信号中准确估计动作电位。复杂的神经元放电，如破裂和有节奏地活跃的神经元所显示的活动，代表了基于钙信号重建尖峰序列的更大挑战。提出了一种基于信息理论的盲钙信号反卷积方法。该模型旨在最大化非线性滤波器的输出熵，其中非线性由尖峰信号的累积分布函数定义。我们使用龙虾嗅觉器官的嗅觉受体神经元（伯恩斯）来测试我们的最大熵（ME）算法。ME算法的优点是可以只根据尖峰信号的统计量在线训练滤波器，而不需要对表征尖峰和钙信号之间关系的未知传递函数进行任何假设。研究表明，与其他方法相比，ME方法能够更准确地重建爆发的第一个和最后一个尖峰的时间，并且与钙信号的直接时间分辨率相比，它的时间精度提高了五倍。
Advances in calcium imaging have enabled studies of the dynamic activity of both individual neurons and neuronal assemblies. However, challenges, such as unknown nonlinearities in the spike–calcium relationship, noise, and the often relatively low temporal resolution of the calcium signal compared to the time-scale of spike generation, restrict the accurate estimation of action potentials from the calcium signal. Complex neuronal discharge, such as the activity demonstrated by bursting and rhythmically active neurons, represents an even greater challenge for reconstructing spike trains based on calcium signals. We propose a method using blind calcium signal deconvolution based on an information-theoretic approach. This model is meant to maximise the output entropy of a nonlinear filter where the nonlinearity is defined by the cumulative distribution function of the spike signal. We tested our maximum entropy (ME) algorithm using bursting olfactory receptor neurons (bORNs) of the lobster olfactory organ. The advantage of the ME algorithm is that the filter can be trained online based only on the statistics of the spike signal, without any assumptions regarding the unknown transfer function characterizing the relation between the spike and calcium signal. We show that the ME method is able to more accurately reconstruct the timing of the first and last spikes of a burst compared to other methods and that it improves the temporal precision fivefold compared to direct timing resolution of calcium signal.
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影响因子：
3.7
作者：
Bobkov Y;Park I;Ukhanov K;Principe J;Ache B
通讯作者：
Ache B
影响因子：
5.4
作者：
Chi, CY;Chen, CH
通讯作者：
Chen, CH
影响因子：
--
作者：
PARZEN, E
通讯作者：
PARZEN, E
影响因子：
6.8
作者：
BENVENISTE, A;GOURSAT, M;RUGET, G
通讯作者：
RUGET, G
影响因子：
3.4
作者：
Helmchen, F;Imoto, K;Sakmann, B
通讯作者：
Sakmann, B