A quantitative study on the photothermal effect of immuno gold nanocages targeted to breast cancer cells.
A quantitative study on the photothermal effect of immuno gold nanocages targeted to breast cancer cells.
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DOI：
10.1021/nn800370j
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发表时间：
2008-08
期刊：
影响因子：
17.1
通讯作者：
Xia, Younan
中科院分区：
文献类型：
--
作者：
Au, Leslie;Zheng, Desheng;Zhou, Fei;Li, Zhi-Yuan;Li, Xingde;Xia, Younan
关键词：
利用平均边长为65±7 nm、800 nm处有强吸收峰的金纳米笼与单克隆抗体（抗HER2）偶联，通过细胞表面过表达的表皮生长因子受体（HER2）靶向乳腺癌细胞（SK-BR-3）。采用流式细胞术定量测定每个细胞固定免疫金纳米笼的数量和光热治疗效果。用脉冲近红外激光照射靶细胞，通过改变功率密度、激光照射时间和照射后反应时间，我们能够优化治疗条件，以达到有效破坏癌细胞的目的。我们发现，免疫金纳米笼靶向的细胞对激光照射立即产生反应，并且当功率密度大于1.6 W/cm2时，细胞损伤是不可逆的。随着暴露时间的增加，死亡细胞的比例逐渐增加，直至5 min后趋于稳定。通过量化免疫金纳米笼的光热效应，获得了关于纳米笼的最佳剂量和激光照射参数的关键信息，并将应用于未来的体内研究。
Gold nanocages with an average edge length of 65 ± 7 nm and a strong absorption peak at 800 nm were conjugated with monoclonal antibodies (anti-HER2) to target breast cancer cells (SK-BR-3) through the epidermal growth factor receptor (in this case, HER2), which is overexpressed on the surfaces of the cells. Both the number of immuno Au nanocages immobilized per cell and the photothermal therapeutic effect were quantified using flow cytometry. The targeted cells were irradiated with a pulsed near-infrared laser, and by varying the power density, the duration of laser exposure, and the time of response after irradiation, we were able to optimize the treatment conditions to achieve effective destruction of the cancer cells. We found that cells targeted with the immuno Au nanocages responded immediately to laser irradiation and that the cellular damage was irreversible at power densities greater than 1.6 W/cm2. The percentage of dead cells increased with increasing exposure time up to 5 min and then became steady. By quantifying the photothermal effect of immuno Au nanocages, critical information with regards to both the optimal dosage of nanocages and parameters of the laser irradiation has been garnered and will be applied to future in vivo studies.
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影响因子：
2.8
作者：
Siekkinen, Andrew R.;McLellan, Joseph M.;Xia, Younan
通讯作者：
Xia, Younan
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
影响因子：
10.8
作者：
Chen, Jingyi;Wang, Danling;Li, Xingde
通讯作者：
Li, Xingde
影响因子：
56.9
作者：
Sun, YG;Xia, YN
通讯作者：
Xia, YN
DOI：
10.1073/pnas.92.8.3353
发表时间：
1995-04-11
影响因子：
11.1
作者：
HURWITZ, E;STANCOVSKI, I;YARDEN, Y
通讯作者：
YARDEN, Y