The Drosophila MAST kinase Drop out is required to initiate membrane compartmentalisation during cellularisation and regulates dynein-based transport.
The Drosophila MAST kinase Drop out is required to initiate membrane compartmentalisation during cellularisation and regulates dynein-based transport.
复制标题
DOI：
10.1242/dev.104711
复制
发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Müller HA
中科院分区：
文献类型：
--
作者：
Hain D;Langlands A;Sonnenberg HC;Bailey C;Bullock SL;Müller HA
果蝇合胞胚囊胚化为极化胚泡上皮提供了一个很好的模型，用来确定发育过程中皮质质膜的不对称是如何产生的。驱动细胞化的分子机制的许多组成部分已经被识别，但在膜不对称开始时作用的细胞信号事件却知之甚少。在这里，我们表明，在早期细胞化中，脱落(DOP)突变扰乱了膜皮质隔室的分离和E-钙粘附素聚集到基础黏附连接中。DOP是正常皱纹形成所必需的，它控制着皱纹通道蛋白的紧密定位和早期皱纹上F-肌动蛋白焦点的形成。我们发现DOP编码微管相关的丝氨酸/苏氨酸(MAST)酶的单一果蝇同源物。DOP与动力蛋白/动力蛋白复合体的成分在基因上相互作用，并促进依赖动力蛋白的胚胎转运。DOP功能的丧失减少了Dynein中间链的磷酸化，提示DOP通过直接或间接机制参与调节细胞质Dynein的活性。这些数据表明DOP通过对细胞质动力蛋白的发育调节来影响沟纹形成的启动。
Cellularisation of the Drosophila syncytial blastoderm embryo into the polarised blastoderm epithelium provides an excellent model with which to determine how cortical plasma membrane asymmetry is generated during development. Many components of the molecular machinery driving cellularisation have been identified, but cell signalling events acting at the onset of membrane asymmetry are poorly understood. Here we show that mutations in drop out (dop) disturb the segregation of membrane cortical compartments and the clustering of E-cadherin into basal adherens junctions in early cellularisation. dop is required for normal furrow formation and controls the tight localisation of furrow canal proteins and the formation of F-actin foci at the incipient furrows. We show that dop encodes the single Drosophila homologue of microtubule-associated Ser/Thr (MAST) kinases. dop interacts genetically with components of the dynein/dynactin complex and promotes dynein-dependent transport in the embryo. Loss of dop function reduces phosphorylation of Dynein intermediate chain, suggesting that dop is involved in regulating cytoplasmic dynein activity through direct or indirect mechanisms. These data suggest that Dop impinges upon the initiation of furrow formation through developmental regulation of cytoplasmic dynein.
登录
查看更多内容
Dynein介导的体内货物运输。开关控制旅行距离。
影响因子：
7.8
作者：
Gross, S P;Welte, M A;Block, S M;Wieschaus, E F
通讯作者：
Wieschaus, E F
影响因子：
3.7
作者：
Hain D;Bettencourt BR;Okamura K;Csorba T;Meyer W;Jin Z;Biggerstaff J;Siomi H;Hutvagner G;Lai EC;Welte M;Müller HA
通讯作者：
Müller HA
MAST3：一种调节TLR4信号传导的新型IBD风险因素。
影响因子：
5
作者：
Labbe, C.;Goyette, P.;Lefebvre, C.;Stevens, C.;Green, T.;Tello-Ruiz, M. K.;Cao, Z.;Landry, A. L.;Stempak, J.;Annese, V.;Latiano, A.;Brant, S. R.;Duerr, R. H.;Taylor, K. D.;Cho, J. H.;Steinhart, A. H.;Daly, M. J.;Silverberg, M. S.;Xavier, R. J.;Rioux, J. D.
通讯作者：
Rioux, J. D.
影响因子：
4.5
作者：
Meyer WJ;Schreiber S;Guo Y;Volkmann T;Welte MA;Müller HA
通讯作者：
Müller HA
影响因子：
11.8
作者：
Lecuit, T;Samanta, R;Wieschaus, E
通讯作者：
Wieschaus, E