Salt stress alters membrane lipid content and lipid biosynthesis pathways in the plasma membrane and tonoplast.
Salt stress alters membrane lipid content and lipid biosynthesis pathways in the plasma membrane and tonoplast.
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DOI：
10.1093/plphys/kiac123
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发表时间：
2022-06-01
期刊：
影响因子：
7.4
通讯作者：
Barkla, Bronwyn J.
中科院分区：
文献类型：
--
作者：
Guo, Qi;Liu, Lei;Rupasinghe, Thusitha W. T.;Roessner, Ute;Barkla, Bronwyn J.
植物细胞膜是感知和启动对包括盐分胁迫在内的环境因子变化的快速反应的场所。了解膜重塑的机制对于研究植物的耐盐性具有重要意义。由于次优的细胞膜分离技术，这项任务在复杂的组织中仍然具有挑战性。在这里，我们利用了一种基于表面电荷的分离方法，即自由流动电泳法，从盐生植物冰草(Mesenanhemum Crystal allum L.)的叶片组织中分离出液泡膜(TP)和质膜(PM)。结果表明，在盐分条件下，该植物发生了膜特异性的脂体重塑，包括TP中形成双层的脂质磷脂酰胆碱的比例增加，PM中非双层形成和带负电荷的脂质(磷脂酰乙醇胺和磷脂酰丝氨酸)增加定量蛋白质组学显示，盐诱导了与脂肪酸合成和脱饱和、甘油脂和甾醇合成有关的蛋白质以及参与脂质信号、结合和运输的蛋白质的变化。这些结果揭示了植物膜动态平衡的一个基本机制，其中响应盐胁迫的脂体重塑有助于维持单个亚细胞隔室的生理功能。基于电荷的膜分离技术和串联质谱仪结合蛋白质组和脂组学方法揭示了植物在盐胁迫下的膜特异性脂质重塑。
Plant cell membranes are the sites of sensing and initiation of rapid responses to changing environmental factors including salinity stress. Understanding the mechanisms involved in membrane remodeling is important for studying salt tolerance in plants. This task remains challenging in complex tissue due to suboptimal subcellular membrane isolation techniques. Here, we capitalized on the use of a surface charge-based separation method, free flow electrophoresis, to isolate the tonoplast (TP) and plasma membrane (PM) from leaf tissue of the halophyte ice plant (Mesembryanthemum crystallinum L.). Results demonstrated a membrane-specific lipidomic remodeling in this plant under salt conditions, including an increased proportion of bilayer forming lipid phosphatidylcholine in the TP and an increase in nonbilayer forming and negatively charged lipids (phosphatidylethanolamine and phosphatidylserine) in the PM. Quantitative proteomics showed salt-induced changes in proteins involved in fatty acid synthesis and desaturation, glycerolipid, and sterol synthesis, as well as proteins involved in lipid signaling, binding, and trafficking. These results reveal an essential plant mechanism for membrane homeostasis wherein lipidome remodeling in response to salt stress contributes to maintaining the physiological function of individual subcellular compartments. Charge-based membrane fractionation techniques and tandem mass spectrometry combined with proteomic and lipidomic approaches reveal membrane-specific lipid remodeling in plants during salt stress.
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影响因子：
4.6
作者：
Dlouhy, Ondrej;Kurasova, Irena;Garab, Gyozo
通讯作者：
Garab, Gyozo
影响因子：
11.6
作者：
Barkla, Bronwyn J.;Vera-Estrella, Rosario;Pantoja, Omar
通讯作者：
Pantoja, Omar
影响因子：
2.5
作者：
Chalbi, Najla;Hessini, Kamel;Ben Youssef, Nabil
通讯作者：
Ben Youssef, Nabil
Na（+）/H（+）交换的细胞内pH调节需要磷脂酰肌醇4,5-双磷酸。
DOI：
10.1083/jcb.150.1.213
发表时间：
2000-07-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Aharonovitz O;Zaun HC;Balla T;York JD;Orlowski J;Grinstein S
通讯作者：
Grinstein S
影响因子：
2.9
作者：
Brown MF
通讯作者：
Brown MF