ATP and autophosphorylation driven conformational changes of HipA kinase revealed by ion mobility and crosslinking mass spectrometry
ATP and autophosphorylation driven conformational changes of HipA kinase revealed by ion mobility and crosslinking mass spectrometry
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离子淌度和交联质谱揭示 ATP 和自磷酸化驱动的 HipA 激酶构象变化
DOI：
10.1007/s00216-016-9709-3
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发表时间：
2016-06
影响因子：
4.3
通讯作者：
Devreese Bart
中科院分区：
文献类型：
--
作者：
Wen Yurong;Sobott Frank;Devreese Bart
毒素-抗毒素系统是广泛参与细菌细胞过程的遗传模块，包括持久性、多药耐药性和耐受性、生物膜形成和发病机制。在II型毒素-抗毒素系统中，毒素和抗毒素都是蛋白质。在原型大肠杆菌HipA-HipB模块中，抗毒素HipB与蛋白激酶HipA形成复合体，并将其隔离在类核中。HIPA不再能够磷酸化谷氨酰-tRNA合成酶，这阻止了即将到来的严格反应的启动。在这里，我们利用天然电喷雾离子迁移率-质谱法和化学交联-质谱法研究了单氏希瓦氏菌MR-1 HipA-HipB复合体的组装。我们揭示了HipA的自动磷酸化伴随着一个大的构象变化，并证实了结构证据。一个相同的MR-1 HipA-HipB组装不同于原型E。HipA-HipB复合体。图形抽象流动质谱仪显示从非结构化HipA到紧密折叠的磷酸化蛋白的两个相变
Toxin-antitoxin systems are genetic modules involved in a broad range of bacterial cellular processes including persistence, multidrug resistance and tolerance, biofilm formation, and pathogenesis. In type II toxin-antitoxin systems, both the toxin and antitoxin are proteins. In the prototypicEscherichia coliHipA-HipB module, the antitoxin HipB forms a complex with the protein kinase HipA and sequesters it in the nucleoid. HipA is then no longer able to phosphorylate glutamyl-tRNA-synthetase and this prevents the initiation of the forthcoming stringent response. Here we investigated the assembly of theShewanella oneidensisMR-1 HipA-HipB complex using native electrospray ion mobility-mass spectrometry and chemical crosslinking combined with mass spectrometry. We revealed that the HipA autophosphorylation was accompanied by a large conformational change, and confirmed structural evidence thatS. oneidensisMR-1 HipA-HipB assembly was distinct from the prototypicE. coliHipA-HipB complex.Graphical abstractIon mobility mass spectrometry shows a two phase transition from unstructured HipA to a compact folded phosphorylated protein
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影响因子：
2.6
作者：
S. Theunissen;L. De Smet;A. Dansercoer;B. Motte;T. Coenye;J. V. Van Beeumen;B. Devreese;S. Savvides;B. Vergauwen
通讯作者：
S. Theunissen;L. De Smet;A. Dansercoer;B. Motte;T. Coenye;J. V. Van Beeumen;B. Devreese;S. Savvides;B. Vergauwen
影响因子：
4.8
作者：
De Gieter, Steven;Konijnenberg, Albert;Garcia-Pino, Abel
通讯作者：
Garcia-Pino, Abel
影响因子：
6.8
作者：
Benesch JL;Ruotolo BT
通讯作者：
Ruotolo BT
影响因子：
16
作者：
Germain, Elsa;Castro-Roa, Daniel;Gerdes, Kenn
通讯作者：
Gerdes, Kenn
影响因子：
16.6
作者：
Kaspy, Ilana;Rotem, Eitan;Glaser, Gad
通讯作者：
Glaser, Gad