Circulating IgA, IgG, and IgM class antibody against Haemophilus parainfluenzae antigens in patients with IgA nephropathy
Circulating IgA, IgG, and IgM class antibody against Haemophilus parainfluenzae antigens in patients with IgA nephropathy
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IgA 肾病患者抗副流感嗜血杆菌抗原的循环 IgA、IgG 和 IgM 类抗体
DOI：
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发表时间：
1996
影响因子：
4.6
通讯作者：
M. Arakawa
中科院分区：
文献类型：
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作者：
S. Suzuki;Y. Nakatomi;S. Odani;H. Sato;F. Gejyo;M. Arakawa
我们之前证明了副流感嗜血杆菌（HP）抗原外膜（OMHP）和IgA肾病（IgAN）之间的密切关系。我们的目的是通过ELISA澄清44例IgAN患者和62例其他肾小球疾病（OGD）患者血清中抗OMHP的IgA、IgG和IgM类抗体之间的关系。IgAN患者抗OMHP的IgA抗体（P < 0.0005）和IgG抗体（P < 0.001）水平明显高于OGD患者。IgAN患者血清中抗OMHP的IgA抗体与IgG抗体、IgA抗体与IgM抗体、IgG抗体与IgM抗体呈正相关。免疫印迹显示，在所有IgAN患者的血清中，针对OMHP的IgA、IgG或IgM抗体与OMHP的成分结合。IgAN患者血清中识别的OMHP的三个组分的氨基酸序列显示与流感嗜血杆菌外膜蛋白（OMP） P6前体、OMP P5和P2孔蛋白的氨基酸序列同源。结果表明，IgAN患者在肾小球中沉积了HP的OMP P6前体、OMP P5或P2孔蛋白，并且通过对这些蛋白的多克隆激活特异性地增加了针对OMHP的IgA抗体的产生，并从一种同型转换到另一种，例如从IgM转换到IgA。
We previously demonstrated a close relationship between the outer membranes of Haemophilus parainfluenzae (HP) antigens (OMHP) and IgA nephrophathy (IgAN). Our objective was to clarify the relationship among IgA, IgG, and IgM class antibody against OMHP in the sera of 44 patients with IgAN and 62 patients with other glomerular diseases (OGD) by ELISA. Patients with IgAN showed a significantly higher level of IgA antibodies (P < 0.0005) and IgG antibodies (P < 0.001) against OMHP, than did patients with OGD. Positive correlations were observed between IgA and IgG antibodies, between IgA and IgM antibodies, and between IgG and IgM antibodies against OMHP in the sera of patients with IgAN. Immunoblotting showed that IgA, IgG, or IgM antibodies against OHMP in the sera of all patients with IgAN bound to the components of OMHP. Amino acid sequences of three components of OMHP recognized by the sera from patients with IgAN revealed homology with those reported for outer membrane protein (OMP) P6 precursor, OMP P5, and P2 porin protein of H. influenzae. Results suggest that patients with IgAN have glomerular deposits of OMP P6 precursor, OMP P5, or P2 porin protein of HP, and a specific increase in the production of IgA antibodies against OMHP via polyclonal activation against these, with switching of production from one isotype to another, e.g. from IgM to IgA.
影响因子：
15.9
作者：
CONLEY, ME;COOPER, MD;MICHAEL, AF
通讯作者：
MICHAEL, AF