Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum.
Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum.
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DOI：
10.1016/j.intimp.2013.01.015
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发表时间：
2013-03
影响因子：
5.6
通讯作者：
Quinn MT
中科院分区：
文献类型：
--
作者：
Schepetkin IA;Kouakou K;Yapi A;Kirpotina LN;Jutila MA;Quinn MT
在历史上，风车子属的不同物种的叶的提取物已被用于治疗各种医学问题。然而，很少有人知道的活性成分赋予这些提取物的治疗特性。本研究对总状风柴叶多糖的生化特性和免疫调节活性进行了评价。水溶性多糖的提取方法。总状花序提取和分级的DEAE-纤维素和Diaion HP-20，以获得Diaion结合的馏分，命名为Combretum多糖-酸性结合或CP-AB，其用甲醇洗脱，和未结合的馏分，命名为CP-AU。进行了分子量测定、糖分析和馏分的其他物理和化学表征。级分CP-AU（mol.重量5.0 kDa）含有II型阿拉伯半乳聚糖，具有强效免疫调节活性，可诱导人外周血单核细胞（PBMC）和MonoMac-6单核细胞产生白细胞介素（IL）-1β、-6、-10和肿瘤坏死因子-α（TNF-α）。同样地，腹膜内施用CP-AU增加了小鼠体内IL-6和单核细胞趋化蛋白-1（MCP-1）的血清水平。Toll样受体4（TLR 4）拮抗剂LPS-RS可抑制CP-AU诱导的PBMC分泌TNF-α。用CP-AU处理诱导Akt 2、Akt 3、GSK-3β、HSP 27、mTOR和所有p38 MAPK亚型（α、β、δ和γ）的磷酸化，以及AP-1/NF-κB转录活性的刺激。此外，CP-AU有效地凝集来自几个物种的红细胞，包括人、小鼠和兔。相比之下，组分CP-AB在所有生物学试验中无活性，包括细胞因子产生和血凝。这些数据表明，至少部分的有益的治疗效果报道的叶水提取物从C。总状花序是由于白细胞功能的调节。
Extracts of leaves of different species of the genus Combretum have been used historically to treat a variety of medicinal problems. However, little is known about the active components conferring therapeutic properties to these extracts. In the present studies, we evaluated biochemical properties and immunomodulatory activity of polysaccharides isolated from the leaves of Combretum racemosum. Water-soluble polysaccharides from leaves of C. racemosum were extracted and fractionated by DEAE-cellulose and Diaion HP-20 to obtain a Diaion-bound fraction, designated Combretum polysaccharide-acidic bound or CP-AB, which was eluted with methanol, and an unbound fraction, designated as CP-AU. Molecular weight determination, sugar analysis, and other physical and chemical characterization of the fractions were performed. Fraction CP-AU (mol. weight 5.0 kDa) contained type II arabinogalactan and had potent immunomodulatory activity, inducing the production of interleukin (IL)-1β, -6, -10, and tumor necrosis factor-α (TNF-α) by human peripheral blood mononuclear cells (PBMC) and MonoMac-6 monocytic cells. Likewise, intraperitoneal administration of CP-AU increased in vivo serum levels of IL-6 and monocyte chemoattractant protein-1 (MCP-1) in mice. CP-AU-induced secretion of TNF-α in PBMC was prevented by Toll-like receptor 4 (TLR4) antagonist LPS-RS. Treatment with CP-AU induced phosphorylation of Akt2, Akt3, GSK-3β, HSP27, mTOR, and all p38 MAPK isoforms (α, β, δ, and γ), as well as stimulation of AP-1/NF-κB transcriptional activity. In addition, CP-AU effectively agglutinated erythrocytes from several species, including human, mouse, and rabbit. In contrast, fraction CP-AB was inactive in all biological tests, including cytokine production and hemagglutination. These data suggest that at least part of the beneficial therapeutic effects reported for the water extracts of leaves from C. racemosum are due to modulation of leukocyte functions.
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Combretum属（Combretaceae）的生物活性：评论。
DOI：
10.3390/molecules17089142
发表时间：
2012-08-02
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
de Morais Lima GR;de Sales IR;Caldas Filho MR;de Jesus NZ;de Sousa Falcão H;Barbosa-Filho JM;Cabral AG;Souto AL;Tavares JF;Batista LM
通讯作者：
Batista LM
影响因子：
3.7
作者：
Holderness J;Schepetkin IA;Freedman B;Kirpotina LN;Quinn MT;Hedges JF;Jutila MA
通讯作者：
Jutila MA
影响因子：
3
作者：
Go, Hiroe;Hwang, Hye-Jung;Taek-Jeong Nam
通讯作者：
Taek-Jeong Nam
影响因子：
3.3
作者：
Ben Sghaier, Mohamed;Krifa, Mounira;Chekir-Ghedira, Leila
通讯作者：
Chekir-Ghedira, Leila
影响因子：
4.4
作者：
Hwang, Insun;Ahn, Ginnae;Jee, Youngheun
通讯作者：
Jee, Youngheun