Polysaccharides isolated from Açaí fruit induce innate immune responses.
Polysaccharides isolated from Açaí fruit induce innate immune responses.
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DOI：
10.1371/journal.pone.0017301
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发表时间：
2011-02-28
期刊：
影响因子：
3.7
通讯作者：
Jutila MA
中科院分区：
文献类型：
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作者：
Holderness J;Schepetkin IA;Freedman B;Kirpotina LN;Quinn MT;Hedges JF;Jutila MA
Açaí（巴西莓）水果是一种流行的营养补充剂，据称可以增强免疫系统功能。这些传闻的说法得到了有限的研究的支持，这些研究描述了巴西莓多酚部分的免疫反应。之前，我们研究了γδ T细胞对几种植物源性营养补充剂中多酚和多糖组分的反应。巴西莓果实中也含有类似的多酚和多糖。因此，我们假设这些部分中的一个或两个可以激活γδ T细胞。与之前的报道相反，我们没有在多酚部分中发现激动剂活性；然而，巴西莓多糖部分在人、小鼠和牛PBMC培养中诱导了强大的γδ T细胞刺激活性。为了表征对巴西莓多糖的免疫反应，我们对粗多糖进行了分离，并在人PBMC培养中测试了这些组分的活性。最大的巴西莓多糖在体外对髓细胞和γδ T细胞的活化作用最强。在体内传递时，巴西莓多糖诱导髓细胞募集和IL-12的产生。这些结果定义了巴西莓多糖成分诱导的先天免疫反应，并对哮喘和传染病的治疗具有启示意义。
The Açaí (Acai) fruit is a popular nutritional supplement that purportedly enhances immune system function. These anecdotal claims are supported by limited studies describing immune responses to the Acai polyphenol fraction. Previously, we characterized γδ T cell responses to both polyphenol and polysaccharide fractions from several plant-derived nutritional supplements. Similar polyphenol and polysaccharide fractions are found in Acai fruit. Thus, we hypothesized that one or both of these fractions could activate γδ T cells. Contrary to previous reports, we did not identify agonist activity in the polyphenol fraction; however, the Acai polysaccharide fraction induced robust γδ T cell stimulatory activity in human, mouse, and bovine PBMC cultures. To characterize the immune response to Acai polysaccharides, we fractionated the crude polysaccharide preparation and tested these fractions for activity in human PBMC cultures. The largest Acai polysaccharides were the most active in vitro as indicated by activation of myeloid and γδ T cells. When delivered in vivo, Acai polysaccharide induced myeloid cell recruitment and IL-12 production. These results define innate immune responses induced by the polysaccharide component of Acai and have implications for the treatment of asthma and infectious disease.
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影响因子：
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